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Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation

Cells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promi...

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Detalles Bibliográficos
Autores principales: Lai, Yinzhi, Asthana, Amish, Cheng, Ke, Kisaalita, William S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203927/
https://www.ncbi.nlm.nih.gov/pubmed/22046371
http://dx.doi.org/10.1371/journal.pone.0026821
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author Lai, Yinzhi
Asthana, Amish
Cheng, Ke
Kisaalita, William S.
author_facet Lai, Yinzhi
Asthana, Amish
Cheng, Ke
Kisaalita, William S.
author_sort Lai, Yinzhi
collection PubMed
description Cells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promising alternative for use in cell-based assays or biosensors in non-clinical drug discovery studies. To advance 3D culture technology, a case has been made for identifying and validating three-dimensionality biomarkers. With this goal in mind, we conducted a transcriptomic expression comparison among neural progenitor cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and as 3D neurospheres (in vivo surrogate). Up-regulation of cytokines as a group in 3D and neurospheres was observed. A group of 13 cytokines were commonly up-regulated in cells cultured in polystyrene scaffolds and neurospheres, suggesting potential for any or a combination from this list to serve as three-dimensionality biomarkers. These results are supportive of further cytokine identification and validation studies with cells from non-neural tissue.
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spelling pubmed-32039272011-11-01 Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation Lai, Yinzhi Asthana, Amish Cheng, Ke Kisaalita, William S. PLoS One Research Article Cells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promising alternative for use in cell-based assays or biosensors in non-clinical drug discovery studies. To advance 3D culture technology, a case has been made for identifying and validating three-dimensionality biomarkers. With this goal in mind, we conducted a transcriptomic expression comparison among neural progenitor cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and as 3D neurospheres (in vivo surrogate). Up-regulation of cytokines as a group in 3D and neurospheres was observed. A group of 13 cytokines were commonly up-regulated in cells cultured in polystyrene scaffolds and neurospheres, suggesting potential for any or a combination from this list to serve as three-dimensionality biomarkers. These results are supportive of further cytokine identification and validation studies with cells from non-neural tissue. Public Library of Science 2011-10-28 /pmc/articles/PMC3203927/ /pubmed/22046371 http://dx.doi.org/10.1371/journal.pone.0026821 Text en Lai et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lai, Yinzhi
Asthana, Amish
Cheng, Ke
Kisaalita, William S.
Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title_full Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title_fullStr Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title_full_unstemmed Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title_short Neural Cell 3D Microtissue Formation Is Marked by Cytokines' Up-Regulation
title_sort neural cell 3d microtissue formation is marked by cytokines' up-regulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203927/
https://www.ncbi.nlm.nih.gov/pubmed/22046371
http://dx.doi.org/10.1371/journal.pone.0026821
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