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The Mitochondrial Genome of Baylisascaris procyonis
BACKGROUND: Baylisascaris procyonis (Nematoda: Ascaridida), an intestinal nematode of raccoons, is emerging as an important helminthic zoonosis due to serious or fatal larval migrans in animals and humans. Despite its significant veterinary and public health impact, the epidemiology, molecular ecolo...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203944/ https://www.ncbi.nlm.nih.gov/pubmed/22046447 http://dx.doi.org/10.1371/journal.pone.0027066 |
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author | Xie, Yue Zhang, Zhihe Niu, Lili Wang, Qiang Wang, Chengdong Lan, Jingchao Deng, Jiabo Fu, Yan Nie, Huaming Yan, Ning Yang, Deying Hao, Guiying Gu, Xiaobin Wang, Shuxian Peng, Xuerong Yang, Guangyou |
author_facet | Xie, Yue Zhang, Zhihe Niu, Lili Wang, Qiang Wang, Chengdong Lan, Jingchao Deng, Jiabo Fu, Yan Nie, Huaming Yan, Ning Yang, Deying Hao, Guiying Gu, Xiaobin Wang, Shuxian Peng, Xuerong Yang, Guangyou |
author_sort | Xie, Yue |
collection | PubMed |
description | BACKGROUND: Baylisascaris procyonis (Nematoda: Ascaridida), an intestinal nematode of raccoons, is emerging as an important helminthic zoonosis due to serious or fatal larval migrans in animals and humans. Despite its significant veterinary and public health impact, the epidemiology, molecular ecology and population genetics of this parasite remain largely unexplored. Mitochondrial (mt) genomes can provide a foundation for investigations in these areas and assist in the diagnosis and control of B. procyonis. In this study, the first complete mt genome sequence of B. procyonis was determined using a polymerase chain reaction (PCR)-based primer-walking strategy. METHODOLOGY/PRINCIPAL FINDINGS: The circular mt genome (14781 bp) of B. procyonis contained 12 protein-coding, 22 transfer RNA and 2 ribosomal RNA genes congruent with other chromadorean nematodes. Interestingly, the B. procyonis mtDNA featured an extremely long AT-rich region (1375 bp) and a high number of intergenic spacers (17), making it unique compared with other secernentean nematodes characterized to date. Additionally, the entire genome displayed notable levels of AT skew and GC skew. Based on pairwise comparisons and sliding window analysis of mt genes among the available 11 Ascaridida mtDNAs, new primer pairs were designed to amplify specific short fragments of the genes cytb (548 bp fragment) and rrnL (200 bp fragment) in the B. procyonis mtDNA, and tested as possible alternatives to existing mt molecular beacons for Ascaridida. Finally, phylogenetic analysis of mtDNAs provided novel estimates of the interrelationships of Baylisasaris and Ascaridida. CONCLUSIONS/SIGNIFICANCE: The complete mt genome sequence of B. procyonis sequenced here should contribute to molecular diagnostic methods, epidemiological investigations and ecological studies of B. procyonis and other related ascaridoids. The information will be important in refining the phylogenetic relationships within the order Ascaridida and enriching the resource of markers for systematic, population genetic and evolutionary biological studies of parasitic nematodes of socio-economic importance. |
format | Online Article Text |
id | pubmed-3203944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32039442011-11-01 The Mitochondrial Genome of Baylisascaris procyonis Xie, Yue Zhang, Zhihe Niu, Lili Wang, Qiang Wang, Chengdong Lan, Jingchao Deng, Jiabo Fu, Yan Nie, Huaming Yan, Ning Yang, Deying Hao, Guiying Gu, Xiaobin Wang, Shuxian Peng, Xuerong Yang, Guangyou PLoS One Research Article BACKGROUND: Baylisascaris procyonis (Nematoda: Ascaridida), an intestinal nematode of raccoons, is emerging as an important helminthic zoonosis due to serious or fatal larval migrans in animals and humans. Despite its significant veterinary and public health impact, the epidemiology, molecular ecology and population genetics of this parasite remain largely unexplored. Mitochondrial (mt) genomes can provide a foundation for investigations in these areas and assist in the diagnosis and control of B. procyonis. In this study, the first complete mt genome sequence of B. procyonis was determined using a polymerase chain reaction (PCR)-based primer-walking strategy. METHODOLOGY/PRINCIPAL FINDINGS: The circular mt genome (14781 bp) of B. procyonis contained 12 protein-coding, 22 transfer RNA and 2 ribosomal RNA genes congruent with other chromadorean nematodes. Interestingly, the B. procyonis mtDNA featured an extremely long AT-rich region (1375 bp) and a high number of intergenic spacers (17), making it unique compared with other secernentean nematodes characterized to date. Additionally, the entire genome displayed notable levels of AT skew and GC skew. Based on pairwise comparisons and sliding window analysis of mt genes among the available 11 Ascaridida mtDNAs, new primer pairs were designed to amplify specific short fragments of the genes cytb (548 bp fragment) and rrnL (200 bp fragment) in the B. procyonis mtDNA, and tested as possible alternatives to existing mt molecular beacons for Ascaridida. Finally, phylogenetic analysis of mtDNAs provided novel estimates of the interrelationships of Baylisasaris and Ascaridida. CONCLUSIONS/SIGNIFICANCE: The complete mt genome sequence of B. procyonis sequenced here should contribute to molecular diagnostic methods, epidemiological investigations and ecological studies of B. procyonis and other related ascaridoids. The information will be important in refining the phylogenetic relationships within the order Ascaridida and enriching the resource of markers for systematic, population genetic and evolutionary biological studies of parasitic nematodes of socio-economic importance. Public Library of Science 2011-10-28 /pmc/articles/PMC3203944/ /pubmed/22046447 http://dx.doi.org/10.1371/journal.pone.0027066 Text en Xie et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xie, Yue Zhang, Zhihe Niu, Lili Wang, Qiang Wang, Chengdong Lan, Jingchao Deng, Jiabo Fu, Yan Nie, Huaming Yan, Ning Yang, Deying Hao, Guiying Gu, Xiaobin Wang, Shuxian Peng, Xuerong Yang, Guangyou The Mitochondrial Genome of Baylisascaris procyonis |
title | The Mitochondrial Genome of Baylisascaris procyonis
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title_full | The Mitochondrial Genome of Baylisascaris procyonis
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title_fullStr | The Mitochondrial Genome of Baylisascaris procyonis
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title_full_unstemmed | The Mitochondrial Genome of Baylisascaris procyonis
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title_short | The Mitochondrial Genome of Baylisascaris procyonis
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title_sort | mitochondrial genome of baylisascaris procyonis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203944/ https://www.ncbi.nlm.nih.gov/pubmed/22046447 http://dx.doi.org/10.1371/journal.pone.0027066 |
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