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CARM1 Mediates Modulation of Sox2

Sox2 is a key component of the transcription factor network that maintains the pluripotent state of embryonic stem cells (ESCs). Sox2 is regulated by multiple post-translational modifications, including ubiquitination, sumoylation, acetylation and phosphorylation. Here we report that Sox2 is in asso...

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Autores principales: Zhao, Hai-yong, Zhang, Yan-jun, Dai, Hui, Zhang, Ye, Shen, Yu-fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203945/
https://www.ncbi.nlm.nih.gov/pubmed/22046437
http://dx.doi.org/10.1371/journal.pone.0027026
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author Zhao, Hai-yong
Zhang, Yan-jun
Dai, Hui
Zhang, Ye
Shen, Yu-fei
author_facet Zhao, Hai-yong
Zhang, Yan-jun
Dai, Hui
Zhang, Ye
Shen, Yu-fei
author_sort Zhao, Hai-yong
collection PubMed
description Sox2 is a key component of the transcription factor network that maintains the pluripotent state of embryonic stem cells (ESCs). Sox2 is regulated by multiple post-translational modifications, including ubiquitination, sumoylation, acetylation and phosphorylation. Here we report that Sox2 is in association with and methylated by coactivator-associated arginine methyltransferase 1 (CARM1), a protein arginine methyltransferase that plays a pivotal role in ESCs. We found that CARM1 facilitates Sox2-mediated transactivation and directly methylates Sox2 at arginine 113. This methylation event enhances Sox2 self-association. Furthermore, the physiological retention of Sox2 on chromatin restricts the Sox2 methylation level. Our study reveals the direct regulation of Sox2 by CARM1 that sheds lights on how arginine methylation signals are integrated into the pluripotent transcription factor network.
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spelling pubmed-32039452011-11-01 CARM1 Mediates Modulation of Sox2 Zhao, Hai-yong Zhang, Yan-jun Dai, Hui Zhang, Ye Shen, Yu-fei PLoS One Research Article Sox2 is a key component of the transcription factor network that maintains the pluripotent state of embryonic stem cells (ESCs). Sox2 is regulated by multiple post-translational modifications, including ubiquitination, sumoylation, acetylation and phosphorylation. Here we report that Sox2 is in association with and methylated by coactivator-associated arginine methyltransferase 1 (CARM1), a protein arginine methyltransferase that plays a pivotal role in ESCs. We found that CARM1 facilitates Sox2-mediated transactivation and directly methylates Sox2 at arginine 113. This methylation event enhances Sox2 self-association. Furthermore, the physiological retention of Sox2 on chromatin restricts the Sox2 methylation level. Our study reveals the direct regulation of Sox2 by CARM1 that sheds lights on how arginine methylation signals are integrated into the pluripotent transcription factor network. Public Library of Science 2011-10-28 /pmc/articles/PMC3203945/ /pubmed/22046437 http://dx.doi.org/10.1371/journal.pone.0027026 Text en Zhao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhao, Hai-yong
Zhang, Yan-jun
Dai, Hui
Zhang, Ye
Shen, Yu-fei
CARM1 Mediates Modulation of Sox2
title CARM1 Mediates Modulation of Sox2
title_full CARM1 Mediates Modulation of Sox2
title_fullStr CARM1 Mediates Modulation of Sox2
title_full_unstemmed CARM1 Mediates Modulation of Sox2
title_short CARM1 Mediates Modulation of Sox2
title_sort carm1 mediates modulation of sox2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203945/
https://www.ncbi.nlm.nih.gov/pubmed/22046437
http://dx.doi.org/10.1371/journal.pone.0027026
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