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Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics

Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is e...

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Autores principales: Lomakin, Alexis J., Kraikivski, Pavel, Semenova, Irina, Ikeda, Kazuho, Zaliapin, Ilya, Tirnauer, Jennifer S., Akhmanova, Anna, Rodionov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204065/
https://www.ncbi.nlm.nih.gov/pubmed/21880898
http://dx.doi.org/10.1091/mbc.E11-03-0260
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author Lomakin, Alexis J.
Kraikivski, Pavel
Semenova, Irina
Ikeda, Kazuho
Zaliapin, Ilya
Tirnauer, Jennifer S.
Akhmanova, Anna
Rodionov, Vladimir
author_facet Lomakin, Alexis J.
Kraikivski, Pavel
Semenova, Irina
Ikeda, Kazuho
Zaliapin, Ilya
Tirnauer, Jennifer S.
Akhmanova, Anna
Rodionov, Vladimir
author_sort Lomakin, Alexis J.
collection PubMed
description Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is enriched at growing MT plus ends. Here we used Xenopus melanophores to test whether signals that stimulate minus end MT transport also enhance CLIP-170–dependent binding of melanosomes to MT tips. We found that these signals significantly (>twofold) increased the number of growing MT plus ends and their density at the cell periphery, thereby enhancing the likelihood of interaction with dispersed melanosomes. Computational simulations showed that local and global increases in the density of CLIP-170–decorated MT plus ends could reduce the half-time of melanosome aggregation by ∼50%. We conclude that pigment granule aggregation signals in melanophores stimulate MT minus end–directed transport by the increasing number of growing MT plus ends decorated with CLIP-170 and redistributing these ends to more efficiently capture melanosomes throughout the cytoplasm.
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spelling pubmed-32040652012-01-16 Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics Lomakin, Alexis J. Kraikivski, Pavel Semenova, Irina Ikeda, Kazuho Zaliapin, Ilya Tirnauer, Jennifer S. Akhmanova, Anna Rodionov, Vladimir Mol Biol Cell Articles Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is enriched at growing MT plus ends. Here we used Xenopus melanophores to test whether signals that stimulate minus end MT transport also enhance CLIP-170–dependent binding of melanosomes to MT tips. We found that these signals significantly (>twofold) increased the number of growing MT plus ends and their density at the cell periphery, thereby enhancing the likelihood of interaction with dispersed melanosomes. Computational simulations showed that local and global increases in the density of CLIP-170–decorated MT plus ends could reduce the half-time of melanosome aggregation by ∼50%. We conclude that pigment granule aggregation signals in melanophores stimulate MT minus end–directed transport by the increasing number of growing MT plus ends decorated with CLIP-170 and redistributing these ends to more efficiently capture melanosomes throughout the cytoplasm. The American Society for Cell Biology 2011-11-01 /pmc/articles/PMC3204065/ /pubmed/21880898 http://dx.doi.org/10.1091/mbc.E11-03-0260 Text en © 2011 Lomakin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Lomakin, Alexis J.
Kraikivski, Pavel
Semenova, Irina
Ikeda, Kazuho
Zaliapin, Ilya
Tirnauer, Jennifer S.
Akhmanova, Anna
Rodionov, Vladimir
Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title_full Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title_fullStr Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title_full_unstemmed Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title_short Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
title_sort stimulation of the clip-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204065/
https://www.ncbi.nlm.nih.gov/pubmed/21880898
http://dx.doi.org/10.1091/mbc.E11-03-0260
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