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Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics
Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is e...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204065/ https://www.ncbi.nlm.nih.gov/pubmed/21880898 http://dx.doi.org/10.1091/mbc.E11-03-0260 |
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author | Lomakin, Alexis J. Kraikivski, Pavel Semenova, Irina Ikeda, Kazuho Zaliapin, Ilya Tirnauer, Jennifer S. Akhmanova, Anna Rodionov, Vladimir |
author_facet | Lomakin, Alexis J. Kraikivski, Pavel Semenova, Irina Ikeda, Kazuho Zaliapin, Ilya Tirnauer, Jennifer S. Akhmanova, Anna Rodionov, Vladimir |
author_sort | Lomakin, Alexis J. |
collection | PubMed |
description | Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is enriched at growing MT plus ends. Here we used Xenopus melanophores to test whether signals that stimulate minus end MT transport also enhance CLIP-170–dependent binding of melanosomes to MT tips. We found that these signals significantly (>twofold) increased the number of growing MT plus ends and their density at the cell periphery, thereby enhancing the likelihood of interaction with dispersed melanosomes. Computational simulations showed that local and global increases in the density of CLIP-170–decorated MT plus ends could reduce the half-time of melanosome aggregation by ∼50%. We conclude that pigment granule aggregation signals in melanophores stimulate MT minus end–directed transport by the increasing number of growing MT plus ends decorated with CLIP-170 and redistributing these ends to more efficiently capture melanosomes throughout the cytoplasm. |
format | Online Article Text |
id | pubmed-3204065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32040652012-01-16 Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics Lomakin, Alexis J. Kraikivski, Pavel Semenova, Irina Ikeda, Kazuho Zaliapin, Ilya Tirnauer, Jennifer S. Akhmanova, Anna Rodionov, Vladimir Mol Biol Cell Articles Cytoplasmic microtubules (MTs) continuously grow and shorten at their free plus ends, a behavior that allows them to capture membrane organelles destined for MT minus end–directed transport. In Xenopus melanophores, the capture of pigment granules (melanosomes) involves the +TIP CLIP-170, which is enriched at growing MT plus ends. Here we used Xenopus melanophores to test whether signals that stimulate minus end MT transport also enhance CLIP-170–dependent binding of melanosomes to MT tips. We found that these signals significantly (>twofold) increased the number of growing MT plus ends and their density at the cell periphery, thereby enhancing the likelihood of interaction with dispersed melanosomes. Computational simulations showed that local and global increases in the density of CLIP-170–decorated MT plus ends could reduce the half-time of melanosome aggregation by ∼50%. We conclude that pigment granule aggregation signals in melanophores stimulate MT minus end–directed transport by the increasing number of growing MT plus ends decorated with CLIP-170 and redistributing these ends to more efficiently capture melanosomes throughout the cytoplasm. The American Society for Cell Biology 2011-11-01 /pmc/articles/PMC3204065/ /pubmed/21880898 http://dx.doi.org/10.1091/mbc.E11-03-0260 Text en © 2011 Lomakin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Lomakin, Alexis J. Kraikivski, Pavel Semenova, Irina Ikeda, Kazuho Zaliapin, Ilya Tirnauer, Jennifer S. Akhmanova, Anna Rodionov, Vladimir Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title | Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title_full | Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title_fullStr | Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title_full_unstemmed | Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title_short | Stimulation of the CLIP-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
title_sort | stimulation of the clip-170–dependent capture of membrane organelles by microtubules through fine tuning of microtubule assembly dynamics |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204065/ https://www.ncbi.nlm.nih.gov/pubmed/21880898 http://dx.doi.org/10.1091/mbc.E11-03-0260 |
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