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In situ proliferation and differentiation of macrophages in dental pulp
The presence of macrophages in dental pulp is well known. However, whether these macrophages proliferate and differentiate in the dental pulp in situ, or whether they constantly migrate from the blood stream into the dental pulp remains unknown. We have examined and compared the development of denta...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204101/ https://www.ncbi.nlm.nih.gov/pubmed/21922246 http://dx.doi.org/10.1007/s00441-011-1231-5 |
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author | Iwasaki, Yukikatsu Otsuka, Hirotada Yanagisawa, Nobuaki Hisamitsu, Hisashi Manabe, Atsufumi Nonaka, Naoko Nakamura, Masanori |
author_facet | Iwasaki, Yukikatsu Otsuka, Hirotada Yanagisawa, Nobuaki Hisamitsu, Hisashi Manabe, Atsufumi Nonaka, Naoko Nakamura, Masanori |
author_sort | Iwasaki, Yukikatsu |
collection | PubMed |
description | The presence of macrophages in dental pulp is well known. However, whether these macrophages proliferate and differentiate in the dental pulp in situ, or whether they constantly migrate from the blood stream into the dental pulp remains unknown. We have examined and compared the development of dental pulp macrophages in an organ culture system with in vivo tooth organs to clarify the developmental mechanism of these macrophages. The first mandibular molar tooth organs from ICR mice aged between 16 days of gestation (E16) to 5 days postnatally were used for in vivo experiments. Those from E16 were cultured for up to 14 days with or without 10% fetal bovine serum. Dental pulp tissues were analyzed with immunohistochemistry to detect the macrophages and with reverse transcription and the polymerase chain reaction (RT-PCR) for the detection of factors related to macrophage development. The growth curves for the in vivo and in vitro cultured cells revealed similar numbers of F4/80-positive macrophages in the dental pulp. RT-PCR analysis indicated the constant expression of myeloid colony-stimulating factor (M-CSF) in both in-vivo- and in-vitro-cultured dental pulp tissues. Anti-M-CSF antibodies significantly inhibited the increase in the number of macrophages in the dental pulp. These results suggest that (1) most of the dental pulp macrophages proliferate and differentiate in the dental pulp without a supply of precursor cells from the blood stream, (2) M-CSF might be a candidate molecule for dental pulp macrophage development, and (3) serum factors might not directly affect the development of macrophages. |
format | Online Article Text |
id | pubmed-3204101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-32041012011-11-10 In situ proliferation and differentiation of macrophages in dental pulp Iwasaki, Yukikatsu Otsuka, Hirotada Yanagisawa, Nobuaki Hisamitsu, Hisashi Manabe, Atsufumi Nonaka, Naoko Nakamura, Masanori Cell Tissue Res Regular Article The presence of macrophages in dental pulp is well known. However, whether these macrophages proliferate and differentiate in the dental pulp in situ, or whether they constantly migrate from the blood stream into the dental pulp remains unknown. We have examined and compared the development of dental pulp macrophages in an organ culture system with in vivo tooth organs to clarify the developmental mechanism of these macrophages. The first mandibular molar tooth organs from ICR mice aged between 16 days of gestation (E16) to 5 days postnatally were used for in vivo experiments. Those from E16 were cultured for up to 14 days with or without 10% fetal bovine serum. Dental pulp tissues were analyzed with immunohistochemistry to detect the macrophages and with reverse transcription and the polymerase chain reaction (RT-PCR) for the detection of factors related to macrophage development. The growth curves for the in vivo and in vitro cultured cells revealed similar numbers of F4/80-positive macrophages in the dental pulp. RT-PCR analysis indicated the constant expression of myeloid colony-stimulating factor (M-CSF) in both in-vivo- and in-vitro-cultured dental pulp tissues. Anti-M-CSF antibodies significantly inhibited the increase in the number of macrophages in the dental pulp. These results suggest that (1) most of the dental pulp macrophages proliferate and differentiate in the dental pulp without a supply of precursor cells from the blood stream, (2) M-CSF might be a candidate molecule for dental pulp macrophage development, and (3) serum factors might not directly affect the development of macrophages. Springer-Verlag 2011-09-16 2011 /pmc/articles/PMC3204101/ /pubmed/21922246 http://dx.doi.org/10.1007/s00441-011-1231-5 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Regular Article Iwasaki, Yukikatsu Otsuka, Hirotada Yanagisawa, Nobuaki Hisamitsu, Hisashi Manabe, Atsufumi Nonaka, Naoko Nakamura, Masanori In situ proliferation and differentiation of macrophages in dental pulp |
title | In situ proliferation and differentiation of macrophages in dental pulp |
title_full | In situ proliferation and differentiation of macrophages in dental pulp |
title_fullStr | In situ proliferation and differentiation of macrophages in dental pulp |
title_full_unstemmed | In situ proliferation and differentiation of macrophages in dental pulp |
title_short | In situ proliferation and differentiation of macrophages in dental pulp |
title_sort | in situ proliferation and differentiation of macrophages in dental pulp |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204101/ https://www.ncbi.nlm.nih.gov/pubmed/21922246 http://dx.doi.org/10.1007/s00441-011-1231-5 |
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