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Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica

BACKGROUND: Angiosperm mitochondrial genomes are more complex than those of other organisms. Analyses of the mitochondrial genome sequences of at least 11 angiosperm species have showed several common properties; these cannot easily explain, however, how the diverse mitotypes evolved within each gen...

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Autores principales: Chang, Shengxin, Yang, Tiantian, Du, Tongqing, Huang, Yongjuan, Chen, Jianmei, Yan, Jiyong, He, Jianbo, Guan, Rongzhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204307/
https://www.ncbi.nlm.nih.gov/pubmed/21988783
http://dx.doi.org/10.1186/1471-2164-12-497
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author Chang, Shengxin
Yang, Tiantian
Du, Tongqing
Huang, Yongjuan
Chen, Jianmei
Yan, Jiyong
He, Jianbo
Guan, Rongzhan
author_facet Chang, Shengxin
Yang, Tiantian
Du, Tongqing
Huang, Yongjuan
Chen, Jianmei
Yan, Jiyong
He, Jianbo
Guan, Rongzhan
author_sort Chang, Shengxin
collection PubMed
description BACKGROUND: Angiosperm mitochondrial genomes are more complex than those of other organisms. Analyses of the mitochondrial genome sequences of at least 11 angiosperm species have showed several common properties; these cannot easily explain, however, how the diverse mitotypes evolved within each genus or species. We analyzed the evolutionary relationships of Brassica mitotypes by sequencing. RESULTS: We sequenced the mitotypes of cam (Brassica rapa), ole (B. oleracea), jun (B. juncea), and car (B. carinata) and analyzed them together with two previously sequenced mitotypes of B. napus (pol and nap). The sizes of whole single circular genomes of cam, jun, ole, and car are 219,747 bp, 219,766 bp, 360,271 bp, and 232,241 bp, respectively. The mitochondrial genome of ole is largest as a resulting of the duplication of a 141.8 kb segment. The jun mitotype is the result of an inherited cam mitotype, and pol is also derived from the cam mitotype with evolutionary modifications. Genes with known functions are conserved in all mitotypes, but clear variation in open reading frames (ORFs) with unknown functions among the six mitotypes was observed. Sequence relationship analysis showed that there has been genome compaction and inheritance in the course of Brassica mitotype evolution. CONCLUSIONS: We have sequenced four Brassica mitotypes, compared six Brassica mitotypes and suggested a mechanism for mitochondrial genome formation in Brassica, including evolutionary events such as inheritance, duplication, rearrangement, genome compaction, and mutation.
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spelling pubmed-32043072011-10-30 Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica Chang, Shengxin Yang, Tiantian Du, Tongqing Huang, Yongjuan Chen, Jianmei Yan, Jiyong He, Jianbo Guan, Rongzhan BMC Genomics Research Article BACKGROUND: Angiosperm mitochondrial genomes are more complex than those of other organisms. Analyses of the mitochondrial genome sequences of at least 11 angiosperm species have showed several common properties; these cannot easily explain, however, how the diverse mitotypes evolved within each genus or species. We analyzed the evolutionary relationships of Brassica mitotypes by sequencing. RESULTS: We sequenced the mitotypes of cam (Brassica rapa), ole (B. oleracea), jun (B. juncea), and car (B. carinata) and analyzed them together with two previously sequenced mitotypes of B. napus (pol and nap). The sizes of whole single circular genomes of cam, jun, ole, and car are 219,747 bp, 219,766 bp, 360,271 bp, and 232,241 bp, respectively. The mitochondrial genome of ole is largest as a resulting of the duplication of a 141.8 kb segment. The jun mitotype is the result of an inherited cam mitotype, and pol is also derived from the cam mitotype with evolutionary modifications. Genes with known functions are conserved in all mitotypes, but clear variation in open reading frames (ORFs) with unknown functions among the six mitotypes was observed. Sequence relationship analysis showed that there has been genome compaction and inheritance in the course of Brassica mitotype evolution. CONCLUSIONS: We have sequenced four Brassica mitotypes, compared six Brassica mitotypes and suggested a mechanism for mitochondrial genome formation in Brassica, including evolutionary events such as inheritance, duplication, rearrangement, genome compaction, and mutation. BioMed Central 2011-10-11 /pmc/articles/PMC3204307/ /pubmed/21988783 http://dx.doi.org/10.1186/1471-2164-12-497 Text en Copyright ©2011 Chang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chang, Shengxin
Yang, Tiantian
Du, Tongqing
Huang, Yongjuan
Chen, Jianmei
Yan, Jiyong
He, Jianbo
Guan, Rongzhan
Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title_full Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title_fullStr Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title_full_unstemmed Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title_short Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica
title_sort mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in brassica
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204307/
https://www.ncbi.nlm.nih.gov/pubmed/21988783
http://dx.doi.org/10.1186/1471-2164-12-497
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