Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demon...

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Detalles Bibliográficos
Autores principales: Drew, Patrick J., Shih, Andy Y., Driscoll, Jonathan D., Knutsen, Per Magne, Blinder, Pablo, Davalos, Dimitrios, Akassoglou, Katerina, Tsai, Philbert S., Kleinfeld, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204312/
https://www.ncbi.nlm.nih.gov/pubmed/20966916
http://dx.doi.org/10.1038/nmeth.1530
Descripción
Sumario:We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demonstrate repeated cortical imaging of dendritic spines, microglia, and angioarchitecture, as well as illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.

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