Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demon...

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Autores principales: Drew, Patrick J., Shih, Andy Y., Driscoll, Jonathan D., Knutsen, Per Magne, Blinder, Pablo, Davalos, Dimitrios, Akassoglou, Katerina, Tsai, Philbert S., Kleinfeld, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204312/
https://www.ncbi.nlm.nih.gov/pubmed/20966916
http://dx.doi.org/10.1038/nmeth.1530
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author Drew, Patrick J.
Shih, Andy Y.
Driscoll, Jonathan D.
Knutsen, Per Magne
Blinder, Pablo
Davalos, Dimitrios
Akassoglou, Katerina
Tsai, Philbert S.
Kleinfeld, David
author_facet Drew, Patrick J.
Shih, Andy Y.
Driscoll, Jonathan D.
Knutsen, Per Magne
Blinder, Pablo
Davalos, Dimitrios
Akassoglou, Katerina
Tsai, Philbert S.
Kleinfeld, David
author_sort Drew, Patrick J.
collection PubMed
description We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demonstrate repeated cortical imaging of dendritic spines, microglia, and angioarchitecture, as well as illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.
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spelling pubmed-32043122011-10-30 Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull Drew, Patrick J. Shih, Andy Y. Driscoll, Jonathan D. Knutsen, Per Magne Blinder, Pablo Davalos, Dimitrios Akassoglou, Katerina Tsai, Philbert S. Kleinfeld, David Nat Methods Article We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demonstrate repeated cortical imaging of dendritic spines, microglia, and angioarchitecture, as well as illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers. 2010-10-21 2010-12 /pmc/articles/PMC3204312/ /pubmed/20966916 http://dx.doi.org/10.1038/nmeth.1530 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Drew, Patrick J.
Shih, Andy Y.
Driscoll, Jonathan D.
Knutsen, Per Magne
Blinder, Pablo
Davalos, Dimitrios
Akassoglou, Katerina
Tsai, Philbert S.
Kleinfeld, David
Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title_full Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title_fullStr Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title_full_unstemmed Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title_short Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
title_sort chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204312/
https://www.ncbi.nlm.nih.gov/pubmed/20966916
http://dx.doi.org/10.1038/nmeth.1530
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