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In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer
PURPOSE: The purpose of this study was to investigate the reliability and the clinical applicability of the adenosine-triphosphate-based chemotherapy response assay (ATP-CRA) as a method of determining in vitro chemosensitivity in patients with gastric cancer. MATERIALS AND METHODS: A total of 243 g...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Gastric Cancer Association
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204504/ https://www.ncbi.nlm.nih.gov/pubmed/22076180 http://dx.doi.org/10.5230/jgc.2010.10.4.155 |
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author | Park, Seulkee Woo, Yanghee Kim, Hogeun Lee, Yong Chan Choi, Sungho Hyung, Woo Jin Noh, Sung Hoon |
author_facet | Park, Seulkee Woo, Yanghee Kim, Hogeun Lee, Yong Chan Choi, Sungho Hyung, Woo Jin Noh, Sung Hoon |
author_sort | Park, Seulkee |
collection | PubMed |
description | PURPOSE: The purpose of this study was to investigate the reliability and the clinical applicability of the adenosine-triphosphate-based chemotherapy response assay (ATP-CRA) as a method of determining in vitro chemosensitivity in patients with gastric cancer. MATERIALS AND METHODS: A total of 243 gastric cancer tissue samples were obtained from gastrectomies performed between February 2007 and January 2010. We evaluated the effectiveness of the ATP-CRA assay in determining the chemosensitivity of gastric cancer specimens using eleven chemotherapeutic agents - etoposide, doxorubicin, epirubicin, mytomicin, 5-fluorouracil, oxaliplatin, irinotecan, docetaxel, paclitaxel, methotraxate, and cisplatin - for chemosensitivity studies using ATP-CRA. We assessed the failure rate, the cell death rate, and the chemosensitivity index. RESULTS: The failure rate of ATP-CRA was 1.6% (4/243). The mean coefficient of variation for triplicate ATP measurements was 6.5%. Etoposide showed the highest cell death rate (35.9%) while methotrexate showed the lowest (16.6%). The most active chemotherapeutic agent was etoposide, which most frequently ranked highest in the chemosensitivity test: 31.9% (51/160). Oxaliplatin was more active against early gastric cancers than advanced gastric cancers, whereas docetaxel was more active against advanced cancers. The lymph node negative group showed a significantly higher cell death rate than the lymph node positive group when treated with doxorubicin, epirubicin, and mitomycin. CONCLUSIONS: ATP-CRA is a stable and clinically applicable in vitro chemosensitivity test with a low failure rate. The clinical usefulness of ATP-CRA should be evaluated by prospective studies comparing the regimen guided by ATP-CRA with an empirical regimen. |
format | Online Article Text |
id | pubmed-3204504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Korean Gastric Cancer Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-32045042011-11-10 In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer Park, Seulkee Woo, Yanghee Kim, Hogeun Lee, Yong Chan Choi, Sungho Hyung, Woo Jin Noh, Sung Hoon J Gastric Cancer Original Article PURPOSE: The purpose of this study was to investigate the reliability and the clinical applicability of the adenosine-triphosphate-based chemotherapy response assay (ATP-CRA) as a method of determining in vitro chemosensitivity in patients with gastric cancer. MATERIALS AND METHODS: A total of 243 gastric cancer tissue samples were obtained from gastrectomies performed between February 2007 and January 2010. We evaluated the effectiveness of the ATP-CRA assay in determining the chemosensitivity of gastric cancer specimens using eleven chemotherapeutic agents - etoposide, doxorubicin, epirubicin, mytomicin, 5-fluorouracil, oxaliplatin, irinotecan, docetaxel, paclitaxel, methotraxate, and cisplatin - for chemosensitivity studies using ATP-CRA. We assessed the failure rate, the cell death rate, and the chemosensitivity index. RESULTS: The failure rate of ATP-CRA was 1.6% (4/243). The mean coefficient of variation for triplicate ATP measurements was 6.5%. Etoposide showed the highest cell death rate (35.9%) while methotrexate showed the lowest (16.6%). The most active chemotherapeutic agent was etoposide, which most frequently ranked highest in the chemosensitivity test: 31.9% (51/160). Oxaliplatin was more active against early gastric cancers than advanced gastric cancers, whereas docetaxel was more active against advanced cancers. The lymph node negative group showed a significantly higher cell death rate than the lymph node positive group when treated with doxorubicin, epirubicin, and mitomycin. CONCLUSIONS: ATP-CRA is a stable and clinically applicable in vitro chemosensitivity test with a low failure rate. The clinical usefulness of ATP-CRA should be evaluated by prospective studies comparing the regimen guided by ATP-CRA with an empirical regimen. The Korean Gastric Cancer Association 2010-12 2010-12-31 /pmc/articles/PMC3204504/ /pubmed/22076180 http://dx.doi.org/10.5230/jgc.2010.10.4.155 Text en Copyright © 2010 by The Korean Gastric Cancer Association http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Seulkee Woo, Yanghee Kim, Hogeun Lee, Yong Chan Choi, Sungho Hyung, Woo Jin Noh, Sung Hoon In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title | In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title_full | In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title_fullStr | In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title_full_unstemmed | In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title_short | In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer |
title_sort | in vitro adenosine triphosphate based chemotherapy response assay in gastric cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204504/ https://www.ncbi.nlm.nih.gov/pubmed/22076180 http://dx.doi.org/10.5230/jgc.2010.10.4.155 |
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