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Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips

Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus...

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Autores principales: Shulman, Lester M., Silberstein, Ilana, Alfandari, Jacqueline, Mendelson, Ella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204647/
https://www.ncbi.nlm.nih.gov/pubmed/21192853
http://dx.doi.org/10.3201/eid1701.101132
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author Shulman, Lester M.
Silberstein, Ilana
Alfandari, Jacqueline
Mendelson, Ella
author_facet Shulman, Lester M.
Silberstein, Ilana
Alfandari, Jacqueline
Mendelson, Ella
author_sort Shulman, Lester M.
collection PubMed
description Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities.
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spelling pubmed-32046472011-11-03 Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips Shulman, Lester M. Silberstein, Ilana Alfandari, Jacqueline Mendelson, Ella Emerg Infect Dis Research Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005–06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities. Centers for Disease Control and Prevention 2011-01 /pmc/articles/PMC3204647/ /pubmed/21192853 http://dx.doi.org/10.3201/eid1701.101132 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Shulman, Lester M.
Silberstein, Ilana
Alfandari, Jacqueline
Mendelson, Ella
Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title_full Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title_fullStr Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title_full_unstemmed Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title_short Genotyping Rotavirus RNA from Archived Rotavirus-Positive Rapid Test Strips
title_sort genotyping rotavirus rna from archived rotavirus-positive rapid test strips
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204647/
https://www.ncbi.nlm.nih.gov/pubmed/21192853
http://dx.doi.org/10.3201/eid1701.101132
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