Cargando…
Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204756/ https://www.ncbi.nlm.nih.gov/pubmed/21291593 http://dx.doi.org/10.3201/eid1702.100620 |
_version_ | 1782215255782326272 |
---|---|
author | Engelthaler, David M. Chiller, Tom Schupp, James A. Colvin, Joshua Beckstrom-Sternberg, Stephen M. Driebe, Elizabeth M. Moses, Tracy Tembe, Waibhav Sinari, Shripad Beckstrom-Sternberg, James S. Christoforides, Alexis Pearson, John V. Carpten, John Keim, Paul Peterson, Ashley Terashita, Dawn Balajee, S. Arunmozhi |
author_facet | Engelthaler, David M. Chiller, Tom Schupp, James A. Colvin, Joshua Beckstrom-Sternberg, Stephen M. Driebe, Elizabeth M. Moses, Tracy Tembe, Waibhav Sinari, Shripad Beckstrom-Sternberg, James S. Christoforides, Alexis Pearson, John V. Carpten, John Keim, Paul Peterson, Ashley Terashita, Dawn Balajee, S. Arunmozhi |
author_sort | Engelthaler, David M. |
collection | PubMed |
description | Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source. |
format | Online Article Text |
id | pubmed-3204756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-32047562011-11-03 Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation Engelthaler, David M. Chiller, Tom Schupp, James A. Colvin, Joshua Beckstrom-Sternberg, Stephen M. Driebe, Elizabeth M. Moses, Tracy Tembe, Waibhav Sinari, Shripad Beckstrom-Sternberg, James S. Christoforides, Alexis Pearson, John V. Carpten, John Keim, Paul Peterson, Ashley Terashita, Dawn Balajee, S. Arunmozhi Emerg Infect Dis Research Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source. Centers for Disease Control and Prevention 2011-02 /pmc/articles/PMC3204756/ /pubmed/21291593 http://dx.doi.org/10.3201/eid1702.100620 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Engelthaler, David M. Chiller, Tom Schupp, James A. Colvin, Joshua Beckstrom-Sternberg, Stephen M. Driebe, Elizabeth M. Moses, Tracy Tembe, Waibhav Sinari, Shripad Beckstrom-Sternberg, James S. Christoforides, Alexis Pearson, John V. Carpten, John Keim, Paul Peterson, Ashley Terashita, Dawn Balajee, S. Arunmozhi Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title | Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title_full | Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title_fullStr | Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title_full_unstemmed | Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title_short | Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
title_sort | next-generation sequencing of coccidioides immitis isolated during cluster investigation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204756/ https://www.ncbi.nlm.nih.gov/pubmed/21291593 http://dx.doi.org/10.3201/eid1702.100620 |
work_keys_str_mv | AT engelthalerdavidm nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT chillertom nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT schuppjamesa nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT colvinjoshua nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT beckstromsternbergstephenm nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT driebeelizabethm nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT mosestracy nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT tembewaibhav nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT sinarishripad nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT beckstromsternbergjamess nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT christoforidesalexis nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT pearsonjohnv nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT carptenjohn nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT keimpaul nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT petersonashley nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT terashitadawn nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation AT balajeesarunmozhi nextgenerationsequencingofcoccidioidesimmitisisolatedduringclusterinvestigation |