Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR i...

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Detalles Bibliográficos
Autores principales: Faux, Cassandra E., Arden, Katherine E., Lambert, Stephen B., Nissen, Michael D., Nolan, Terry M., Chang, Anne B., Sloots, Theo P., Mackay, Ian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2011
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204776/
https://www.ncbi.nlm.nih.gov/pubmed/21291610
http://dx.doi.org/10.3201/eid1702.101123
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author Faux, Cassandra E.
Arden, Katherine E.
Lambert, Stephen B.
Nissen, Michael D.
Nolan, Terry M.
Chang, Anne B.
Sloots, Theo P.
Mackay, Ian M.
author_facet Faux, Cassandra E.
Arden, Katherine E.
Lambert, Stephen B.
Nissen, Michael D.
Nolan, Terry M.
Chang, Anne B.
Sloots, Theo P.
Mackay, Ian M.
author_sort Faux, Cassandra E.
collection PubMed
description We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
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spelling pubmed-32047762011-11-03 Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection Faux, Cassandra E. Arden, Katherine E. Lambert, Stephen B. Nissen, Michael D. Nolan, Terry M. Chang, Anne B. Sloots, Theo P. Mackay, Ian M. Emerg Infect Dis Dispatch We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology. Centers for Disease Control and Prevention 2011-02 /pmc/articles/PMC3204776/ /pubmed/21291610 http://dx.doi.org/10.3201/eid1702.101123 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Faux, Cassandra E.
Arden, Katherine E.
Lambert, Stephen B.
Nissen, Michael D.
Nolan, Terry M.
Chang, Anne B.
Sloots, Theo P.
Mackay, Ian M.
Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title_full Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title_fullStr Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title_full_unstemmed Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title_short Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
title_sort usefulness of published pcr primers in detecting human rhinovirus infection
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204776/
https://www.ncbi.nlm.nih.gov/pubmed/21291610
http://dx.doi.org/10.3201/eid1702.101123
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