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A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms

BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficie...

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Autores principales: Casulli, Sarah, Topçu, Selma, Fattoum, Lakhdar, von Gunten, Stephan, Simon, Hans-Uwe, Teillaud, Jean-Luc, Bayry, Jagadeesh, Kaveri, Srini V., Elbim, Carole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204983/
https://www.ncbi.nlm.nih.gov/pubmed/22065996
http://dx.doi.org/10.1371/journal.pone.0026469
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author Casulli, Sarah
Topçu, Selma
Fattoum, Lakhdar
von Gunten, Stephan
Simon, Hans-Uwe
Teillaud, Jean-Luc
Bayry, Jagadeesh
Kaveri, Srini V.
Elbim, Carole
author_facet Casulli, Sarah
Topçu, Selma
Fattoum, Lakhdar
von Gunten, Stephan
Simon, Hans-Uwe
Teillaud, Jean-Luc
Bayry, Jagadeesh
Kaveri, Srini V.
Elbim, Carole
author_sort Casulli, Sarah
collection PubMed
description BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders. METHODOLOGY/PRINCIPAL FINDINGS: We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation. CONCLUSIONS/SIGNIFICANCE: IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent.
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spelling pubmed-32049832011-11-07 A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms Casulli, Sarah Topçu, Selma Fattoum, Lakhdar von Gunten, Stephan Simon, Hans-Uwe Teillaud, Jean-Luc Bayry, Jagadeesh Kaveri, Srini V. Elbim, Carole PLoS One Research Article BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders. METHODOLOGY/PRINCIPAL FINDINGS: We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation. CONCLUSIONS/SIGNIFICANCE: IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent. Public Library of Science 2011-10-31 /pmc/articles/PMC3204983/ /pubmed/22065996 http://dx.doi.org/10.1371/journal.pone.0026469 Text en Casulli et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Casulli, Sarah
Topçu, Selma
Fattoum, Lakhdar
von Gunten, Stephan
Simon, Hans-Uwe
Teillaud, Jean-Luc
Bayry, Jagadeesh
Kaveri, Srini V.
Elbim, Carole
A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title_full A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title_fullStr A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title_full_unstemmed A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title_short A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
title_sort differential concentration-dependent effect of ivig on neutrophil functions: relevance for anti-microbial and anti-inflammatory mechanisms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204983/
https://www.ncbi.nlm.nih.gov/pubmed/22065996
http://dx.doi.org/10.1371/journal.pone.0026469
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