Cargando…
A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms
BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficie...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204983/ https://www.ncbi.nlm.nih.gov/pubmed/22065996 http://dx.doi.org/10.1371/journal.pone.0026469 |
_version_ | 1782215270139428864 |
---|---|
author | Casulli, Sarah Topçu, Selma Fattoum, Lakhdar von Gunten, Stephan Simon, Hans-Uwe Teillaud, Jean-Luc Bayry, Jagadeesh Kaveri, Srini V. Elbim, Carole |
author_facet | Casulli, Sarah Topçu, Selma Fattoum, Lakhdar von Gunten, Stephan Simon, Hans-Uwe Teillaud, Jean-Luc Bayry, Jagadeesh Kaveri, Srini V. Elbim, Carole |
author_sort | Casulli, Sarah |
collection | PubMed |
description | BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders. METHODOLOGY/PRINCIPAL FINDINGS: We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation. CONCLUSIONS/SIGNIFICANCE: IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent. |
format | Online Article Text |
id | pubmed-3204983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32049832011-11-07 A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms Casulli, Sarah Topçu, Selma Fattoum, Lakhdar von Gunten, Stephan Simon, Hans-Uwe Teillaud, Jean-Luc Bayry, Jagadeesh Kaveri, Srini V. Elbim, Carole PLoS One Research Article BACKGROUND: Polymorphonuclear neutrophils (PMN) play a key role in host defences against invading microorganisms but can also potentiate detrimental inflammatory reactions in case of excessive or misdirected responses. Intravenous immunoglobulins (IVIg) are used to treat patients with immune deficiencies and, at higher doses, in autoimmune, allergic and systemic inflammatory disorders. METHODOLOGY/PRINCIPAL FINDINGS: We used flow cytometry to examine the effects of IVIg on PMN functions and survival, using whole-blood conditions in order to avoid artifacts due to isolation procedures. IVIg at low concentrations induced PMN activation, as reflected by decreased L-selectin and increased CD11b expression at the PMN surface, oxidative burst enhancement, and prolonged cell survival. In contrast, IVIg at higher concentrations inhibited LPS-induced CD11b degranulation and oxidative burst priming, and counteracted LPS-induced PMN lifespan prolongation. CONCLUSIONS/SIGNIFICANCE: IVIg appears to have differential, concentration-dependent effects on PMN, possibly supporting the use of IVIg as either an anti-microbial or an anti-inflammatory agent. Public Library of Science 2011-10-31 /pmc/articles/PMC3204983/ /pubmed/22065996 http://dx.doi.org/10.1371/journal.pone.0026469 Text en Casulli et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Casulli, Sarah Topçu, Selma Fattoum, Lakhdar von Gunten, Stephan Simon, Hans-Uwe Teillaud, Jean-Luc Bayry, Jagadeesh Kaveri, Srini V. Elbim, Carole A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title | A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title_full | A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title_fullStr | A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title_full_unstemmed | A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title_short | A Differential Concentration-Dependent Effect of IVIg on Neutrophil Functions: Relevance for Anti-Microbial and Anti-Inflammatory Mechanisms |
title_sort | differential concentration-dependent effect of ivig on neutrophil functions: relevance for anti-microbial and anti-inflammatory mechanisms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3204983/ https://www.ncbi.nlm.nih.gov/pubmed/22065996 http://dx.doi.org/10.1371/journal.pone.0026469 |
work_keys_str_mv | AT casullisarah adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT topcuselma adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT fattoumlakhdar adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT vonguntenstephan adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT simonhansuwe adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT teillaudjeanluc adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT bayryjagadeesh adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT kaverisriniv adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT elbimcarole adifferentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT casullisarah differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT topcuselma differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT fattoumlakhdar differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT vonguntenstephan differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT simonhansuwe differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT teillaudjeanluc differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT bayryjagadeesh differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT kaverisriniv differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms AT elbimcarole differentialconcentrationdependenteffectofivigonneutrophilfunctionsrelevanceforantimicrobialandantiinflammatorymechanisms |