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DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication

BACKGROUND: Japanese encephalitis virus (JEV) is a member of the mosquito-borne Flaviviridae family of viruses that causes human encephalitis. Upon infection of a new host, replication of viral RNA involves not only the viral RNA-dependent RNA polymerase (RdRp), but also host proteins. Host factors...

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Autores principales: Wang, Robert Yung-Liang, Huang, Yu-Ru, Chong, Ka-Man, Hung, Chun-Yu, Ke, Zhi-Long, Chang, Ruey-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205071/
https://www.ncbi.nlm.nih.gov/pubmed/21999493
http://dx.doi.org/10.1186/1743-422X-8-471
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author Wang, Robert Yung-Liang
Huang, Yu-Ru
Chong, Ka-Man
Hung, Chun-Yu
Ke, Zhi-Long
Chang, Ruey-Yi
author_facet Wang, Robert Yung-Liang
Huang, Yu-Ru
Chong, Ka-Man
Hung, Chun-Yu
Ke, Zhi-Long
Chang, Ruey-Yi
author_sort Wang, Robert Yung-Liang
collection PubMed
description BACKGROUND: Japanese encephalitis virus (JEV) is a member of the mosquito-borne Flaviviridae family of viruses that causes human encephalitis. Upon infection of a new host, replication of viral RNA involves not only the viral RNA-dependent RNA polymerase (RdRp), but also host proteins. Host factors involved in JEV replication are not well characterized. RESULTS: We identified Hdj2, a heat-shock protein 40 (Hsp40)/DnaJ homolog, from a mouse brain cDNA library interacting with JEV nonstructural protein 5 (NS5) encoding viral RdRp using yeast two-hybrid system. Specific interaction of Hdj2 with NS5 was confirmed by coimmunoprecipitation and colocalization in JEV-infected cells. Overexpression of Hdj2 in JEV-infected cells led to an increase of RNA synthesis, and the virus titer was elevated approximately 4.5- to 10-fold. Knocking down of Hdj2 by siRNA reduced the virus production significantly. CONCLUSIONS: We conclude that Hdj2 directly associates with JEV NS5 and facilitates viral replication. This study is the first to demonstrate Hdj2 involved in JEV replication, providing insight into a potential therapeutic target and cell-based vaccine development of JEV infection.
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spelling pubmed-32050712011-11-01 DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication Wang, Robert Yung-Liang Huang, Yu-Ru Chong, Ka-Man Hung, Chun-Yu Ke, Zhi-Long Chang, Ruey-Yi Virol J Research BACKGROUND: Japanese encephalitis virus (JEV) is a member of the mosquito-borne Flaviviridae family of viruses that causes human encephalitis. Upon infection of a new host, replication of viral RNA involves not only the viral RNA-dependent RNA polymerase (RdRp), but also host proteins. Host factors involved in JEV replication are not well characterized. RESULTS: We identified Hdj2, a heat-shock protein 40 (Hsp40)/DnaJ homolog, from a mouse brain cDNA library interacting with JEV nonstructural protein 5 (NS5) encoding viral RdRp using yeast two-hybrid system. Specific interaction of Hdj2 with NS5 was confirmed by coimmunoprecipitation and colocalization in JEV-infected cells. Overexpression of Hdj2 in JEV-infected cells led to an increase of RNA synthesis, and the virus titer was elevated approximately 4.5- to 10-fold. Knocking down of Hdj2 by siRNA reduced the virus production significantly. CONCLUSIONS: We conclude that Hdj2 directly associates with JEV NS5 and facilitates viral replication. This study is the first to demonstrate Hdj2 involved in JEV replication, providing insight into a potential therapeutic target and cell-based vaccine development of JEV infection. BioMed Central 2011-10-14 /pmc/articles/PMC3205071/ /pubmed/21999493 http://dx.doi.org/10.1186/1743-422X-8-471 Text en Copyright ©2011 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wang, Robert Yung-Liang
Huang, Yu-Ru
Chong, Ka-Man
Hung, Chun-Yu
Ke, Zhi-Long
Chang, Ruey-Yi
DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title_full DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title_fullStr DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title_full_unstemmed DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title_short DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication
title_sort dnaj homolog hdj2 facilitates japanese encephalitis virus replication
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205071/
https://www.ncbi.nlm.nih.gov/pubmed/21999493
http://dx.doi.org/10.1186/1743-422X-8-471
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