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Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate

Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we descri...

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Detalles Bibliográficos
Autores principales: Velazquez, Francisco, Peak-Chew, Sew Yu, Fernández, Israel S., Neumann, Christopher S., Kay, Robert R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205185/
https://www.ncbi.nlm.nih.gov/pubmed/22035794
http://dx.doi.org/10.1016/j.chembiol.2011.08.003
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author Velazquez, Francisco
Peak-Chew, Sew Yu
Fernández, Israel S.
Neumann, Christopher S.
Kay, Robert R.
author_facet Velazquez, Francisco
Peak-Chew, Sew Yu
Fernández, Israel S.
Neumann, Christopher S.
Kay, Robert R.
author_sort Velazquez, Francisco
collection PubMed
description Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we describe the identification of DIF dechlorinase. DIF dechlorinase is active when expressed in bacteria, and activity is lost from Dictyostelium cells when its gene, drcA, is knocked out. It has a K(m) for DIF of 88 nM and K(cat) of 6.7 s(−1). DrcA is related to glutathione S-transferases, but with a key asparagine-to-cysteine substitution in the catalytic pocket. When this change is reversed, the enzyme reverts to a glutathione S-transferase, thus suggesting a catalytic mechanism. DrcA offers new possibilities for the rational design of bioremediation strategies.
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spelling pubmed-32051852011-12-23 Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate Velazquez, Francisco Peak-Chew, Sew Yu Fernández, Israel S. Neumann, Christopher S. Kay, Robert R. Chem Biol Article Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we describe the identification of DIF dechlorinase. DIF dechlorinase is active when expressed in bacteria, and activity is lost from Dictyostelium cells when its gene, drcA, is knocked out. It has a K(m) for DIF of 88 nM and K(cat) of 6.7 s(−1). DrcA is related to glutathione S-transferases, but with a key asparagine-to-cysteine substitution in the catalytic pocket. When this change is reversed, the enzyme reverts to a glutathione S-transferase, thus suggesting a catalytic mechanism. DrcA offers new possibilities for the rational design of bioremediation strategies. Elsevier 2011-10-28 /pmc/articles/PMC3205185/ /pubmed/22035794 http://dx.doi.org/10.1016/j.chembiol.2011.08.003 Text en © 2011 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Velazquez, Francisco
Peak-Chew, Sew Yu
Fernández, Israel S.
Neumann, Christopher S.
Kay, Robert R.
Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title_full Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title_fullStr Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title_full_unstemmed Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title_short Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
title_sort identification of a eukaryotic reductive dechlorinase and characterization of its mechanism of action on its natural substrate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205185/
https://www.ncbi.nlm.nih.gov/pubmed/22035794
http://dx.doi.org/10.1016/j.chembiol.2011.08.003
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