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Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate
Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we descri...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205185/ https://www.ncbi.nlm.nih.gov/pubmed/22035794 http://dx.doi.org/10.1016/j.chembiol.2011.08.003 |
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author | Velazquez, Francisco Peak-Chew, Sew Yu Fernández, Israel S. Neumann, Christopher S. Kay, Robert R. |
author_facet | Velazquez, Francisco Peak-Chew, Sew Yu Fernández, Israel S. Neumann, Christopher S. Kay, Robert R. |
author_sort | Velazquez, Francisco |
collection | PubMed |
description | Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we describe the identification of DIF dechlorinase. DIF dechlorinase is active when expressed in bacteria, and activity is lost from Dictyostelium cells when its gene, drcA, is knocked out. It has a K(m) for DIF of 88 nM and K(cat) of 6.7 s(−1). DrcA is related to glutathione S-transferases, but with a key asparagine-to-cysteine substitution in the catalytic pocket. When this change is reversed, the enzyme reverts to a glutathione S-transferase, thus suggesting a catalytic mechanism. DrcA offers new possibilities for the rational design of bioremediation strategies. |
format | Online Article Text |
id | pubmed-3205185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-32051852011-12-23 Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate Velazquez, Francisco Peak-Chew, Sew Yu Fernández, Israel S. Neumann, Christopher S. Kay, Robert R. Chem Biol Article Chlorinated compounds are important environmental pollutants whose biodegradation may be limited by inefficient dechlorinating enzymes. Dictyostelium amoebae produce a chlorinated alkyl phenone called DIF which induces stalk cell differentiation during their multicellular development. Here we describe the identification of DIF dechlorinase. DIF dechlorinase is active when expressed in bacteria, and activity is lost from Dictyostelium cells when its gene, drcA, is knocked out. It has a K(m) for DIF of 88 nM and K(cat) of 6.7 s(−1). DrcA is related to glutathione S-transferases, but with a key asparagine-to-cysteine substitution in the catalytic pocket. When this change is reversed, the enzyme reverts to a glutathione S-transferase, thus suggesting a catalytic mechanism. DrcA offers new possibilities for the rational design of bioremediation strategies. Elsevier 2011-10-28 /pmc/articles/PMC3205185/ /pubmed/22035794 http://dx.doi.org/10.1016/j.chembiol.2011.08.003 Text en © 2011 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Velazquez, Francisco Peak-Chew, Sew Yu Fernández, Israel S. Neumann, Christopher S. Kay, Robert R. Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title | Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title_full | Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title_fullStr | Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title_full_unstemmed | Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title_short | Identification of a Eukaryotic Reductive Dechlorinase and Characterization of Its Mechanism of Action on Its Natural Substrate |
title_sort | identification of a eukaryotic reductive dechlorinase and characterization of its mechanism of action on its natural substrate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205185/ https://www.ncbi.nlm.nih.gov/pubmed/22035794 http://dx.doi.org/10.1016/j.chembiol.2011.08.003 |
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