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Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis

Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA...

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Autores principales: Shi, Yan, Sandoghchian Shotorbani, Siamak, Su, Zhaoliang, Liu, Yanfang, Tong, Jia, Zheng, Dong, Chen, Jianguo, Liu, Yingzhao, Xu, Yan, Jiao, Zhijun, Wang, Shengjun, Lu, Liwei, Huang, Xinxiang, Xu, Huaxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205666/
https://www.ncbi.nlm.nih.gov/pubmed/22110531
http://dx.doi.org/10.1155/2012/295081
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author Shi, Yan
Sandoghchian Shotorbani, Siamak
Su, Zhaoliang
Liu, Yanfang
Tong, Jia
Zheng, Dong
Chen, Jianguo
Liu, Yingzhao
Xu, Yan
Jiao, Zhijun
Wang, Shengjun
Lu, Liwei
Huang, Xinxiang
Xu, Huaxi
author_facet Shi, Yan
Sandoghchian Shotorbani, Siamak
Su, Zhaoliang
Liu, Yanfang
Tong, Jia
Zheng, Dong
Chen, Jianguo
Liu, Yingzhao
Xu, Yan
Jiao, Zhijun
Wang, Shengjun
Lu, Liwei
Huang, Xinxiang
Xu, Huaxi
author_sort Shi, Yan
collection PubMed
description Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORγt, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORγt, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.
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spelling pubmed-32056662011-11-22 Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis Shi, Yan Sandoghchian Shotorbani, Siamak Su, Zhaoliang Liu, Yanfang Tong, Jia Zheng, Dong Chen, Jianguo Liu, Yingzhao Xu, Yan Jiao, Zhijun Wang, Shengjun Lu, Liwei Huang, Xinxiang Xu, Huaxi Clin Dev Immunol Research Article Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORγt, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORγt, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients. Hindawi Publishing Corporation 2012 2011-10-26 /pmc/articles/PMC3205666/ /pubmed/22110531 http://dx.doi.org/10.1155/2012/295081 Text en Copyright © 2012 Yan Shi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shi, Yan
Sandoghchian Shotorbani, Siamak
Su, Zhaoliang
Liu, Yanfang
Tong, Jia
Zheng, Dong
Chen, Jianguo
Liu, Yingzhao
Xu, Yan
Jiao, Zhijun
Wang, Shengjun
Lu, Liwei
Huang, Xinxiang
Xu, Huaxi
Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title_full Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title_fullStr Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title_full_unstemmed Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title_short Enhanced HMGB1 Expression May Contribute to Th17 Cells Activation in Rheumatoid Arthritis
title_sort enhanced hmgb1 expression may contribute to th17 cells activation in rheumatoid arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205666/
https://www.ncbi.nlm.nih.gov/pubmed/22110531
http://dx.doi.org/10.1155/2012/295081
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