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Physiological Response to Membrane Protein Overexpression in E. coli
Overexpression represents a principal bottleneck in structural and functional studies of integral membrane proteins (IMPs). Although E. coli remains the leading organism for convenient and economical protein overexpression, many IMPs exhibit toxicity on induction in this host and give low yields of...
Autores principales: | Gubellini, Francesca, Verdon, Grégory, Karpowich, Nathan K., Luff, Jon D., Boël, Grégory, Gauthier, Nils, Handelman, Samuel K., Ades, Sarah E., Hunt, John F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Biochemistry and Molecular Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205863/ https://www.ncbi.nlm.nih.gov/pubmed/21719796 http://dx.doi.org/10.1074/mcp.M111.007930 |
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