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Efficiency of Peptide Nucleic Acid-Directed PCR Clamping and Its Application in the Investigation of Natural Diets of the Japanese Eel Leptocephali

Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis. We investigated PCR-clamping efficiency by studying PNA position and mismatch with complementary DNA by de...

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Detalles Bibliográficos
Autores principales:	Terahara, Takeshi, Chow, Seinen, Kurogi, Hiroaki, Lee, Sun-Hee, Tsukamoto, Katsumi, Mochioka, Noritaka, Tanaka, Hideki, Takeyama, Haruko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206044/ https://www.ncbi.nlm.nih.gov/pubmed/22069444 http://dx.doi.org/10.1371/journal.pone.0025715

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