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Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle

The fission yeast interphase spindle pole body (SPB) is a bipartite structure in which a bulky cytoplasmic domain is separated from a nuclear component by the nuclear envelope. During mitosis, the SPB is incorporated into a fenestra that forms within the envelope during mitotic commitment. Closure o...

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Autores principales: Tamm, Tiina, Grallert, Agnes, Grossman, Emily P.S., Alvarez-Tabares, Isabel, Stevens, Frances E., Hagan, Iain M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206342/
https://www.ncbi.nlm.nih.gov/pubmed/22042620
http://dx.doi.org/10.1083/jcb.201106076
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author Tamm, Tiina
Grallert, Agnes
Grossman, Emily P.S.
Alvarez-Tabares, Isabel
Stevens, Frances E.
Hagan, Iain M.
author_facet Tamm, Tiina
Grallert, Agnes
Grossman, Emily P.S.
Alvarez-Tabares, Isabel
Stevens, Frances E.
Hagan, Iain M.
author_sort Tamm, Tiina
collection PubMed
description The fission yeast interphase spindle pole body (SPB) is a bipartite structure in which a bulky cytoplasmic domain is separated from a nuclear component by the nuclear envelope. During mitosis, the SPB is incorporated into a fenestra that forms within the envelope during mitotic commitment. Closure of this fenestra during anaphase B/mitotic exit returns the cytoplasmic component to the cytoplasmic face of an intact interphase nuclear envelope. Here we show that Brr6 is transiently recruited to SPBs at both SPB insertion and extrusion. Brr6 is required for both SPB insertion and nuclear envelope integrity during anaphase B/mitotic exit. Genetic interactions with apq12 and defective sterol assimilation suggest that Brr6 may alter envelope composition at SPBs to promote SPB insertion and extrusion. The restriction of the Brr6 domain to eukaryotes that use a polar fenestra in an otherwise closed mitosis suggests a conserved role in fenestration to enable a single microtubule organizing center to nucleate both cytoplasmic and nuclear microtubules on opposing sides of the nuclear envelope.
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spelling pubmed-32063422012-04-30 Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle Tamm, Tiina Grallert, Agnes Grossman, Emily P.S. Alvarez-Tabares, Isabel Stevens, Frances E. Hagan, Iain M. J Cell Biol Research Articles The fission yeast interphase spindle pole body (SPB) is a bipartite structure in which a bulky cytoplasmic domain is separated from a nuclear component by the nuclear envelope. During mitosis, the SPB is incorporated into a fenestra that forms within the envelope during mitotic commitment. Closure of this fenestra during anaphase B/mitotic exit returns the cytoplasmic component to the cytoplasmic face of an intact interphase nuclear envelope. Here we show that Brr6 is transiently recruited to SPBs at both SPB insertion and extrusion. Brr6 is required for both SPB insertion and nuclear envelope integrity during anaphase B/mitotic exit. Genetic interactions with apq12 and defective sterol assimilation suggest that Brr6 may alter envelope composition at SPBs to promote SPB insertion and extrusion. The restriction of the Brr6 domain to eukaryotes that use a polar fenestra in an otherwise closed mitosis suggests a conserved role in fenestration to enable a single microtubule organizing center to nucleate both cytoplasmic and nuclear microtubules on opposing sides of the nuclear envelope. The Rockefeller University Press 2011-10-31 /pmc/articles/PMC3206342/ /pubmed/22042620 http://dx.doi.org/10.1083/jcb.201106076 Text en © 2011 Tamm et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Tamm, Tiina
Grallert, Agnes
Grossman, Emily P.S.
Alvarez-Tabares, Isabel
Stevens, Frances E.
Hagan, Iain M.
Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title_full Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title_fullStr Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title_full_unstemmed Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title_short Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
title_sort brr6 drives the schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206342/
https://www.ncbi.nlm.nih.gov/pubmed/22042620
http://dx.doi.org/10.1083/jcb.201106076
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