Cargando…
Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis
BACKGROUND: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H(2)O on osteogenesis. METHODS: Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206489/ https://www.ncbi.nlm.nih.gov/pubmed/21981964 http://dx.doi.org/10.1186/1471-2474-12-227 |
_version_ | 1782215446146056192 |
---|---|
author | Lee, Chiming Cheong, Meileng Hsiao, Wentien Liu, Henyu Tsai, Chingyu Wang, Mingfu Wu, Chihhsiung Chang, Kwanghwa Lam, Gowlin Deng, Winping |
author_facet | Lee, Chiming Cheong, Meileng Hsiao, Wentien Liu, Henyu Tsai, Chingyu Wang, Mingfu Wu, Chihhsiung Chang, Kwanghwa Lam, Gowlin Deng, Winping |
author_sort | Lee, Chiming |
collection | PubMed |
description | BACKGROUND: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H(2)O on osteogenesis. METHODS: Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H(2)O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H(2)O on osteogenesis in vivo. Mice were given either iQPR-H(2)O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. RESULTS: NIH3T3 cells grown in medium prepared with iQPR-H(2)O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H(2)O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H(2)O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H(2)O as compared with control group. CONCLUSIONS: iQPR-H(2)O may reduce the symptoms of osteoporosis by improving osteogenesis. |
format | Online Article Text |
id | pubmed-3206489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32064892011-11-03 Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis Lee, Chiming Cheong, Meileng Hsiao, Wentien Liu, Henyu Tsai, Chingyu Wang, Mingfu Wu, Chihhsiung Chang, Kwanghwa Lam, Gowlin Deng, Winping BMC Musculoskelet Disord Research Article BACKGROUND: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H(2)O on osteogenesis. METHODS: Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H(2)O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H(2)O on osteogenesis in vivo. Mice were given either iQPR-H(2)O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. RESULTS: NIH3T3 cells grown in medium prepared with iQPR-H(2)O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H(2)O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H(2)O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H(2)O as compared with control group. CONCLUSIONS: iQPR-H(2)O may reduce the symptoms of osteoporosis by improving osteogenesis. BioMed Central 2011-10-08 /pmc/articles/PMC3206489/ /pubmed/21981964 http://dx.doi.org/10.1186/1471-2474-12-227 Text en Copyright ©2011 Lee et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lee, Chiming Cheong, Meileng Hsiao, Wentien Liu, Henyu Tsai, Chingyu Wang, Mingfu Wu, Chihhsiung Chang, Kwanghwa Lam, Gowlin Deng, Winping Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title | Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title_full | Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title_fullStr | Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title_full_unstemmed | Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title_short | Use of iQPR-H(2)O for bone regeneration and its potential in the improvement of osteoporosis |
title_sort | use of iqpr-h(2)o for bone regeneration and its potential in the improvement of osteoporosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206489/ https://www.ncbi.nlm.nih.gov/pubmed/21981964 http://dx.doi.org/10.1186/1471-2474-12-227 |
work_keys_str_mv | AT leechiming useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT cheongmeileng useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT hsiaowentien useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT liuhenyu useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT tsaichingyu useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT wangmingfu useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT wuchihhsiung useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT changkwanghwa useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT lamgowlin useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis AT dengwinping useofiqprh2oforboneregenerationanditspotentialintheimprovementofosteoporosis |