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Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility

Similar amounts of N-cadherin and cadherin-7, the prototypes of type I and type II cadherin, induced cell-cell adhesion in murine sarcoma 180 transfectants, Ncad-1 and cad7-29, respectively. However, in the initial phase of aggregation, Ncad-1 cells aggregated more rapidly than cad7-29 cells. Isolat...

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Autores principales: Dufour, Sylvie, Beauvais-Jouneau, Alice, Delouvée, Annie, Thiery, Jean Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206574/
https://www.ncbi.nlm.nih.gov/pubmed/10427101
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author Dufour, Sylvie
Beauvais-Jouneau, Alice
Delouvée, Annie
Thiery, Jean Paul
author_facet Dufour, Sylvie
Beauvais-Jouneau, Alice
Delouvée, Annie
Thiery, Jean Paul
author_sort Dufour, Sylvie
collection PubMed
description Similar amounts of N-cadherin and cadherin-7, the prototypes of type I and type II cadherin, induced cell-cell adhesion in murine sarcoma 180 transfectants, Ncad-1 and cad7-29, respectively. However, in the initial phase of aggregation, Ncad-1 cells aggregated more rapidly than cad7-29 cells. Isolated Ncad-1 and cad7-29 cells adhered and spread in a similar manner on fibronectin (FN), whereas aggregated cad7-29 cells were more motile and dispersed than aggregated Ncad-1 cells. cad7-29 cells established transient contacts with their neighbors which were stabilized if FN-cell interactions were perturbed. In contrast, Ncad-1 cells remained in close contact when they migrated on FN. Both β-catenin and cadherin were more rapidly downregulated in cad7-29 than in Ncad-1 cells treated with cycloheximide, suggesting a higher turnover rate for cadherin-7–mediated cell-cell contacts than for those mediated by N-cadherin. The extent of FN-dependent focal adhesion kinase phosphorylation was much lower if the cells had initiated N-cadherin–mediated rather than cadherin-7–mediated cell adhesion before plating. On grafting into the embryo, Ncad-1 cells did not migrate and remained at or close to the graft site, even after 48 h, whereas grafted cad7-29 cells dispersed efficiently into embryonic structures. Thus, the adhesive phenotype of cadherin-7–expressing cells is regulated by the nature of the extracellular matrix environment which also controls the migratory behavior of the cells. In addition, adhesions mediated by different cadherins differentially regulate FN-dependent signaling. The transient contacts specifically observed in cadherin- 7–expressing cells may also be important in the control of cell motility.
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spelling pubmed-32065742011-11-02 Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility Dufour, Sylvie Beauvais-Jouneau, Alice Delouvée, Annie Thiery, Jean Paul J Cell Biol Original Article Similar amounts of N-cadherin and cadherin-7, the prototypes of type I and type II cadherin, induced cell-cell adhesion in murine sarcoma 180 transfectants, Ncad-1 and cad7-29, respectively. However, in the initial phase of aggregation, Ncad-1 cells aggregated more rapidly than cad7-29 cells. Isolated Ncad-1 and cad7-29 cells adhered and spread in a similar manner on fibronectin (FN), whereas aggregated cad7-29 cells were more motile and dispersed than aggregated Ncad-1 cells. cad7-29 cells established transient contacts with their neighbors which were stabilized if FN-cell interactions were perturbed. In contrast, Ncad-1 cells remained in close contact when they migrated on FN. Both β-catenin and cadherin were more rapidly downregulated in cad7-29 than in Ncad-1 cells treated with cycloheximide, suggesting a higher turnover rate for cadherin-7–mediated cell-cell contacts than for those mediated by N-cadherin. The extent of FN-dependent focal adhesion kinase phosphorylation was much lower if the cells had initiated N-cadherin–mediated rather than cadherin-7–mediated cell adhesion before plating. On grafting into the embryo, Ncad-1 cells did not migrate and remained at or close to the graft site, even after 48 h, whereas grafted cad7-29 cells dispersed efficiently into embryonic structures. Thus, the adhesive phenotype of cadherin-7–expressing cells is regulated by the nature of the extracellular matrix environment which also controls the migratory behavior of the cells. In addition, adhesions mediated by different cadherins differentially regulate FN-dependent signaling. The transient contacts specifically observed in cadherin- 7–expressing cells may also be important in the control of cell motility. The Rockefeller University Press 1999-07-26 /pmc/articles/PMC3206574/ /pubmed/10427101 Text en © 1999 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Dufour, Sylvie
Beauvais-Jouneau, Alice
Delouvée, Annie
Thiery, Jean Paul
Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title_full Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title_fullStr Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title_full_unstemmed Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title_short Differential Function of N-Cadherin and Cadherin-7 in the Control of Embryonic Cell Motility
title_sort differential function of n-cadherin and cadherin-7 in the control of embryonic cell motility
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206574/
https://www.ncbi.nlm.nih.gov/pubmed/10427101
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