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Expression Screening of Fusion Partners from an E. coli Genome for Soluble Expression of Recombinant Proteins in a Cell-Free Protein Synthesis System
While access to soluble recombinant proteins is essential for a number of proteome studies, preparation of purified functional proteins is often limited by the protein solubility. In this study, potent solubility-enhancing fusion partners were screened from the repertoire of endogenous E. coli prote...
Autores principales: | Ahn, Jin-Ho, Keum, Jung-Won, Kim, Dong-Myung |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206877/ https://www.ncbi.nlm.nih.gov/pubmed/22073212 http://dx.doi.org/10.1371/journal.pone.0026875 |
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