Cargando…

Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning

The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Fagman, Henrik, Amendola, Elena, Parrillo, Luca, Zoppoli, Pietro, Marotta, Pina, Scarfò, Marzia, De Luca, Pasquale, de Carvalho, Denise Pires, Ceccarelli, Michele, De Felice, Mario, Di Lauro, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206993/
https://www.ncbi.nlm.nih.gov/pubmed/21924257
http://dx.doi.org/10.1016/j.ydbio.2011.08.015
_version_ 1782215514153549824
author Fagman, Henrik
Amendola, Elena
Parrillo, Luca
Zoppoli, Pietro
Marotta, Pina
Scarfò, Marzia
De Luca, Pasquale
de Carvalho, Denise Pires
Ceccarelli, Michele
De Felice, Mario
Di Lauro, Roberto
author_facet Fagman, Henrik
Amendola, Elena
Parrillo, Luca
Zoppoli, Pietro
Marotta, Pina
Scarfò, Marzia
De Luca, Pasquale
de Carvalho, Denise Pires
Ceccarelli, Michele
De Felice, Mario
Di Lauro, Roberto
author_sort Fagman, Henrik
collection PubMed
description The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.
format Online
Article
Text
id pubmed-3206993
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-32069932012-01-19 Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning Fagman, Henrik Amendola, Elena Parrillo, Luca Zoppoli, Pietro Marotta, Pina Scarfò, Marzia De Luca, Pasquale de Carvalho, Denise Pires Ceccarelli, Michele De Felice, Mario Di Lauro, Roberto Dev Biol Resource The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development. Elsevier 2011-11-15 /pmc/articles/PMC3206993/ /pubmed/21924257 http://dx.doi.org/10.1016/j.ydbio.2011.08.015 Text en © 2011 Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Resource
Fagman, Henrik
Amendola, Elena
Parrillo, Luca
Zoppoli, Pietro
Marotta, Pina
Scarfò, Marzia
De Luca, Pasquale
de Carvalho, Denise Pires
Ceccarelli, Michele
De Felice, Mario
Di Lauro, Roberto
Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title_full Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title_fullStr Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title_full_unstemmed Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title_short Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
title_sort gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206993/
https://www.ncbi.nlm.nih.gov/pubmed/21924257
http://dx.doi.org/10.1016/j.ydbio.2011.08.015
work_keys_str_mv AT fagmanhenrik geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT amendolaelena geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT parrilloluca geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT zoppolipietro geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT marottapina geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT scarfomarzia geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT delucapasquale geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT decarvalhodenisepires geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT ceccarellimichele geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT defelicemario geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning
AT dilauroroberto geneexpressionprofilingatearlyorganogenesisrevealsbothcommonanddiversemechanismsinforegutpatterning