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Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology
Previous studies have demonstrated that microribonucleic acids (miRs) are key regulators of protein expression in the brain and modulate dendritic spine morphology and synaptic activity. To identify novel miRs involved in neuronal plasticity, we exposed adult mice to chronic treatments with nicotine...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207289/ https://www.ncbi.nlm.nih.gov/pubmed/21930776 http://dx.doi.org/10.1083/jcb.201103006 |
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author | Lippi, Giordano Steinert, Joern R. Marczylo, Emma L. D’Oro, Sabina Fiore, Roberto Forsythe, Ian D. Schratt, Gerhard Zoli, Michele Nicotera, Pierluigi Young, Kenneth W. |
author_facet | Lippi, Giordano Steinert, Joern R. Marczylo, Emma L. D’Oro, Sabina Fiore, Roberto Forsythe, Ian D. Schratt, Gerhard Zoli, Michele Nicotera, Pierluigi Young, Kenneth W. |
author_sort | Lippi, Giordano |
collection | PubMed |
description | Previous studies have demonstrated that microribonucleic acids (miRs) are key regulators of protein expression in the brain and modulate dendritic spine morphology and synaptic activity. To identify novel miRs involved in neuronal plasticity, we exposed adult mice to chronic treatments with nicotine, cocaine, or amphetamine, which are psychoactive drugs that induce well-documented neuroadaptations. We observed brain region– and drug-specific changes in miR expression levels and identified miR-29a/b as regulators of synaptic morphology. In vitro imaging experiments indicated that miR-29a/b reduce mushroom-shaped dendritic spines on hippocampal neurons with a concomitant increase in filopodial-like outgrowths, suggesting an effect on synapse formation via actin cytoskeleton remodeling. We identified Arpc3, a component of the ARP2/3 actin nucleation complex, as a bona fide target for down-regulation by miR-29a/b. This work provides evidence that targeting of Arpc3 by miR-29a/b fine tunes structural plasticity by regulating actin network branching in mature and developing spines. |
format | Online Article Text |
id | pubmed-3207289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32072892012-03-19 Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology Lippi, Giordano Steinert, Joern R. Marczylo, Emma L. D’Oro, Sabina Fiore, Roberto Forsythe, Ian D. Schratt, Gerhard Zoli, Michele Nicotera, Pierluigi Young, Kenneth W. J Cell Biol Research Articles Previous studies have demonstrated that microribonucleic acids (miRs) are key regulators of protein expression in the brain and modulate dendritic spine morphology and synaptic activity. To identify novel miRs involved in neuronal plasticity, we exposed adult mice to chronic treatments with nicotine, cocaine, or amphetamine, which are psychoactive drugs that induce well-documented neuroadaptations. We observed brain region– and drug-specific changes in miR expression levels and identified miR-29a/b as regulators of synaptic morphology. In vitro imaging experiments indicated that miR-29a/b reduce mushroom-shaped dendritic spines on hippocampal neurons with a concomitant increase in filopodial-like outgrowths, suggesting an effect on synapse formation via actin cytoskeleton remodeling. We identified Arpc3, a component of the ARP2/3 actin nucleation complex, as a bona fide target for down-regulation by miR-29a/b. This work provides evidence that targeting of Arpc3 by miR-29a/b fine tunes structural plasticity by regulating actin network branching in mature and developing spines. The Rockefeller University Press 2011-09-19 /pmc/articles/PMC3207289/ /pubmed/21930776 http://dx.doi.org/10.1083/jcb.201103006 Text en © 2011 Lippi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Lippi, Giordano Steinert, Joern R. Marczylo, Emma L. D’Oro, Sabina Fiore, Roberto Forsythe, Ian D. Schratt, Gerhard Zoli, Michele Nicotera, Pierluigi Young, Kenneth W. Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title | Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title_full | Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title_fullStr | Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title_full_unstemmed | Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title_short | Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology |
title_sort | targeting of the arpc3 actin nucleation factor by mir-29a/b regulates dendritic spine morphology |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207289/ https://www.ncbi.nlm.nih.gov/pubmed/21930776 http://dx.doi.org/10.1083/jcb.201103006 |
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