Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulato...

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Detalles Bibliográficos
Autores principales: Grover, Ginni, Quirin, Sean, Fiedler, Callie, Piestun, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/
https://www.ncbi.nlm.nih.gov/pubmed/22076263
http://dx.doi.org/10.1364/BOE.2.003010
Descripción
Sumario:We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range.

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