Cargando…
Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulato...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/ https://www.ncbi.nlm.nih.gov/pubmed/22076263 http://dx.doi.org/10.1364/BOE.2.003010 |
_version_ | 1782215541666086912 |
---|---|
author | Grover, Ginni Quirin, Sean Fiedler, Callie Piestun, Rafael |
author_facet | Grover, Ginni Quirin, Sean Fiedler, Callie Piestun, Rafael |
author_sort | Grover, Ginni |
collection | PubMed |
description | We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range. |
format | Online Article Text |
id | pubmed-3207371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-32073712011-11-10 Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging Grover, Ginni Quirin, Sean Fiedler, Callie Piestun, Rafael Biomed Opt Express Microscopy We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range. Optical Society of America 2011-10-05 /pmc/articles/PMC3207371/ /pubmed/22076263 http://dx.doi.org/10.1364/BOE.2.003010 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Microscopy Grover, Ginni Quirin, Sean Fiedler, Callie Piestun, Rafael Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title | Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title_full | Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title_fullStr | Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title_full_unstemmed | Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title_short | Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging |
title_sort | photon efficient double-helix psf microscopy with application to 3d photo-activation localization imaging |
topic | Microscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/ https://www.ncbi.nlm.nih.gov/pubmed/22076263 http://dx.doi.org/10.1364/BOE.2.003010 |
work_keys_str_mv | AT groverginni photonefficientdoublehelixpsfmicroscopywithapplicationto3dphotoactivationlocalizationimaging AT quirinsean photonefficientdoublehelixpsfmicroscopywithapplicationto3dphotoactivationlocalizationimaging AT fiedlercallie photonefficientdoublehelixpsfmicroscopywithapplicationto3dphotoactivationlocalizationimaging AT piestunrafael photonefficientdoublehelixpsfmicroscopywithapplicationto3dphotoactivationlocalizationimaging |