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Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulato...

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Detalles Bibliográficos
Autores principales: Grover, Ginni, Quirin, Sean, Fiedler, Callie, Piestun, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/
https://www.ncbi.nlm.nih.gov/pubmed/22076263
http://dx.doi.org/10.1364/BOE.2.003010
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author Grover, Ginni
Quirin, Sean
Fiedler, Callie
Piestun, Rafael
author_facet Grover, Ginni
Quirin, Sean
Fiedler, Callie
Piestun, Rafael
author_sort Grover, Ginni
collection PubMed
description We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range.
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spelling pubmed-32073712011-11-10 Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging Grover, Ginni Quirin, Sean Fiedler, Callie Piestun, Rafael Biomed Opt Express Microscopy We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range. Optical Society of America 2011-10-05 /pmc/articles/PMC3207371/ /pubmed/22076263 http://dx.doi.org/10.1364/BOE.2.003010 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Microscopy
Grover, Ginni
Quirin, Sean
Fiedler, Callie
Piestun, Rafael
Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title_full Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title_fullStr Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title_full_unstemmed Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title_short Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
title_sort photon efficient double-helix psf microscopy with application to 3d photo-activation localization imaging
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/
https://www.ncbi.nlm.nih.gov/pubmed/22076263
http://dx.doi.org/10.1364/BOE.2.003010
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