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Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging
We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulato...
Autores principales: | Grover, Ginni, Quirin, Sean, Fiedler, Callie, Piestun, Rafael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207371/ https://www.ncbi.nlm.nih.gov/pubmed/22076263 http://dx.doi.org/10.1364/BOE.2.003010 |
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