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Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture

It is valuable to extend genotyping studies of Helicobacter pylori to strains from indigenous communities across the world to better define adaption, evolution, and associated diseases. We aimed to genetically characterize both human individuals and their infecting H. pylori from indigenous communit...

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Autores principales: Camorlinga-Ponce, Margarita, Perez-Perez, Guillermo, Gonzalez-Valencia, Gerardo, Mendoza, Irma, Peñaloza-Espinosa, Rosenda, Ramos, Irma, Kersulyte, Dangeruta, Reyes-Leon, Adriana, Romo, Carolina, Granados, Julio, Muñoz, Leopoldo, Berg, Douglas E., Torres, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207844/
https://www.ncbi.nlm.nih.gov/pubmed/22073291
http://dx.doi.org/10.1371/journal.pone.0027212
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author Camorlinga-Ponce, Margarita
Perez-Perez, Guillermo
Gonzalez-Valencia, Gerardo
Mendoza, Irma
Peñaloza-Espinosa, Rosenda
Ramos, Irma
Kersulyte, Dangeruta
Reyes-Leon, Adriana
Romo, Carolina
Granados, Julio
Muñoz, Leopoldo
Berg, Douglas E.
Torres, Javier
author_facet Camorlinga-Ponce, Margarita
Perez-Perez, Guillermo
Gonzalez-Valencia, Gerardo
Mendoza, Irma
Peñaloza-Espinosa, Rosenda
Ramos, Irma
Kersulyte, Dangeruta
Reyes-Leon, Adriana
Romo, Carolina
Granados, Julio
Muñoz, Leopoldo
Berg, Douglas E.
Torres, Javier
author_sort Camorlinga-Ponce, Margarita
collection PubMed
description It is valuable to extend genotyping studies of Helicobacter pylori to strains from indigenous communities across the world to better define adaption, evolution, and associated diseases. We aimed to genetically characterize both human individuals and their infecting H. pylori from indigenous communities of Mexico, and to compare them with those from other human groups. We studied individuals from three indigenous groups, Tarahumaras from the North, Huichols from the West and Nahuas from the center of Mexico. Volunteers were sampled at their community site, DNA was isolated from white blood cells and mtDNA, Y-chromosome, and STR alleles were studied. H. pylori was cultured from gastric juice, and DNA extracted for genotyping of virulence and housekeeping genes. We found Amerindian mtDNA haplogroups (A, B, C, and D), Y-chromosome DYS19T, and Amerindian STRs alleles frequent in the three groups, confirming Amerindian ancestry in these Mexican groups. Concerning H.pylori cagA phylogenetic analyses, although most isolates were of the Western type, a new Amerindian cluster neither Western nor Asian, was formed by some indigenous Mexican, Colombian, Peruvian and Venezuelan isolates. Similarly, vacA phylogenetic analyses showed the existence of a novel Amerindian type in isolates from Alaska, Mexico and Colombia. With hspA strains from Mexico and other American groups clustered within the three major groups, Asian, African or European. Genotyping of housekeeping genes confirmed that Mexican strains formed a novel Asian-related Amerindian group together with strains from remote Amazon Aborigines. This study shows that Mexican indigenous people with Amerindian markers are colonized with H. pylori showing admixture of Asian, European and African strains in genes known to interact with the gastric mucosa. We present evidence of novel Amerindian cagA and vacA alleles in indigenous groups of North and South America.
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spelling pubmed-32078442011-11-09 Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture Camorlinga-Ponce, Margarita Perez-Perez, Guillermo Gonzalez-Valencia, Gerardo Mendoza, Irma Peñaloza-Espinosa, Rosenda Ramos, Irma Kersulyte, Dangeruta Reyes-Leon, Adriana Romo, Carolina Granados, Julio Muñoz, Leopoldo Berg, Douglas E. Torres, Javier PLoS One Research Article It is valuable to extend genotyping studies of Helicobacter pylori to strains from indigenous communities across the world to better define adaption, evolution, and associated diseases. We aimed to genetically characterize both human individuals and their infecting H. pylori from indigenous communities of Mexico, and to compare them with those from other human groups. We studied individuals from three indigenous groups, Tarahumaras from the North, Huichols from the West and Nahuas from the center of Mexico. Volunteers were sampled at their community site, DNA was isolated from white blood cells and mtDNA, Y-chromosome, and STR alleles were studied. H. pylori was cultured from gastric juice, and DNA extracted for genotyping of virulence and housekeeping genes. We found Amerindian mtDNA haplogroups (A, B, C, and D), Y-chromosome DYS19T, and Amerindian STRs alleles frequent in the three groups, confirming Amerindian ancestry in these Mexican groups. Concerning H.pylori cagA phylogenetic analyses, although most isolates were of the Western type, a new Amerindian cluster neither Western nor Asian, was formed by some indigenous Mexican, Colombian, Peruvian and Venezuelan isolates. Similarly, vacA phylogenetic analyses showed the existence of a novel Amerindian type in isolates from Alaska, Mexico and Colombia. With hspA strains from Mexico and other American groups clustered within the three major groups, Asian, African or European. Genotyping of housekeeping genes confirmed that Mexican strains formed a novel Asian-related Amerindian group together with strains from remote Amazon Aborigines. This study shows that Mexican indigenous people with Amerindian markers are colonized with H. pylori showing admixture of Asian, European and African strains in genes known to interact with the gastric mucosa. We present evidence of novel Amerindian cagA and vacA alleles in indigenous groups of North and South America. Public Library of Science 2011-11-03 /pmc/articles/PMC3207844/ /pubmed/22073291 http://dx.doi.org/10.1371/journal.pone.0027212 Text en Camorlinga-Ponce et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Camorlinga-Ponce, Margarita
Perez-Perez, Guillermo
Gonzalez-Valencia, Gerardo
Mendoza, Irma
Peñaloza-Espinosa, Rosenda
Ramos, Irma
Kersulyte, Dangeruta
Reyes-Leon, Adriana
Romo, Carolina
Granados, Julio
Muñoz, Leopoldo
Berg, Douglas E.
Torres, Javier
Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title_full Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title_fullStr Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title_full_unstemmed Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title_short Helicobacter pylori Genotyping from American Indigenous Groups Shows Novel Amerindian vacA and cagA Alleles and Asian, African and European Admixture
title_sort helicobacter pylori genotyping from american indigenous groups shows novel amerindian vaca and caga alleles and asian, african and european admixture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207844/
https://www.ncbi.nlm.nih.gov/pubmed/22073291
http://dx.doi.org/10.1371/journal.pone.0027212
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