A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling

To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we...

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Detalles Bibliográficos
Autores principales: Fragoso, Variluska, Goddard, Hannah, Baldwin, Ian T, Kim, Sang-Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207920/
https://www.ncbi.nlm.nih.gov/pubmed/22014154
http://dx.doi.org/10.1186/1746-4811-7-34
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author Fragoso, Variluska
Goddard, Hannah
Baldwin, Ian T
Kim, Sang-Gyu
author_facet Fragoso, Variluska
Goddard, Hannah
Baldwin, Ian T
Kim, Sang-Gyu
author_sort Fragoso, Variluska
collection PubMed
description To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed N. attenuata. Our results are consistent with the source-to-sink movement of an sRNA silencing signal.
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spelling pubmed-32079202011-11-04 A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling Fragoso, Variluska Goddard, Hannah Baldwin, Ian T Kim, Sang-Gyu Plant Methods Methodology To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a useful tool for identifying these signals and understanding their functions. Additionally we analyzed transcript accumulation profiles of scions and rootstocks of grafts performed with wild-type and stably transformed N. attenuata. Our results are consistent with the source-to-sink movement of an sRNA silencing signal. BioMed Central 2011-10-20 /pmc/articles/PMC3207920/ /pubmed/22014154 http://dx.doi.org/10.1186/1746-4811-7-34 Text en Copyright ©2011 Fragoso et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Fragoso, Variluska
Goddard, Hannah
Baldwin, Ian T
Kim, Sang-Gyu
A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title_full A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title_fullStr A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title_full_unstemmed A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title_short A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
title_sort simple and efficient micrografting method for stably transformed nicotiana attenuata plants to examine shoot-root signaling
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207920/
https://www.ncbi.nlm.nih.gov/pubmed/22014154
http://dx.doi.org/10.1186/1746-4811-7-34
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