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Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells

Protection of the small intestine from mucosal injury induced by nonsteroidal anti-inflammatory drugs including acetylsalicylic acid is a critical issue in the field of gastroenterology. Polaprezinc an anti-ulcer drug, consisting of zinc and L-carnosine, provides gastric mucosal protection against v...

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Autores principales: Qin, Ying, Naito, Yuji, Handa, Osamu, Hayashi, Natsuko, Kuki, Aiko, Mizushima, Katsura, Omatsu, Tatsushi, Tanimura, Yuko, Morita, Mayuko, Adachi, Satoko, Fukui, Akifumi, Hirata, Ikuhiro, Kishimoto, Etsuko, Nishikawa, Taichiro, Uchiyama, Kazuhiko, Ishikawa, Takeshi, Takagi, Tomohisa, Yagi, Nobuaki, Kokura, Satoshi, Yoshikawa, Toshikazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3208013/
https://www.ncbi.nlm.nih.gov/pubmed/22128216
http://dx.doi.org/10.3164/jcbn.11-26
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author Qin, Ying
Naito, Yuji
Handa, Osamu
Hayashi, Natsuko
Kuki, Aiko
Mizushima, Katsura
Omatsu, Tatsushi
Tanimura, Yuko
Morita, Mayuko
Adachi, Satoko
Fukui, Akifumi
Hirata, Ikuhiro
Kishimoto, Etsuko
Nishikawa, Taichiro
Uchiyama, Kazuhiko
Ishikawa, Takeshi
Takagi, Tomohisa
Yagi, Nobuaki
Kokura, Satoshi
Yoshikawa, Toshikazu
author_facet Qin, Ying
Naito, Yuji
Handa, Osamu
Hayashi, Natsuko
Kuki, Aiko
Mizushima, Katsura
Omatsu, Tatsushi
Tanimura, Yuko
Morita, Mayuko
Adachi, Satoko
Fukui, Akifumi
Hirata, Ikuhiro
Kishimoto, Etsuko
Nishikawa, Taichiro
Uchiyama, Kazuhiko
Ishikawa, Takeshi
Takagi, Tomohisa
Yagi, Nobuaki
Kokura, Satoshi
Yoshikawa, Toshikazu
author_sort Qin, Ying
collection PubMed
description Protection of the small intestine from mucosal injury induced by nonsteroidal anti-inflammatory drugs including acetylsalicylic acid is a critical issue in the field of gastroenterology. Polaprezinc an anti-ulcer drug, consisting of zinc and L-carnosine, provides gastric mucosal protection against various irritants. In this study, we investigated the protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of the RIE1 rat intestinal epithelial cell line. Confluent rat intestinal epithelial cells were incubated with 70 µM polaprezinc for 24 h, and then stimulated with or without 15 mM acetylsalicylic acid for a further 15 h. Subsequent cellular viability was quantified by fluorometric assay based on cell lysis and staining. Acetylsalicylic acid-induced cell death was also qualified by fluorescent microscopy of Hoechst33342 and propidium iodide. Heat shock proteins 70 protein expression after adding polaprezinc or acetylsalicylic acid was assessed by western blotting. To investigate the role of Heat shock protein 70, Heat shock protein 70-specific small interfering RNA was applied. Cell viability was quantified by fluorometric assay based on cell lysis and staining and apoptosis was analyzed by fluorescence-activated cell sorting. We found that acetylsalicylic acid significantly induced apoptosis of rat intestinal epithelial cells in a dose- and time-dependent manner. Polaprezinc significantly suppressed acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells at its late phase. At the same time, polaprezinc increased Heat shock protein 70 expressions of rat intestinal epithelial cells in a time-dependent manner. However, in Heat shock protein 70-silenced rat intestinal epithelial cells, polaprezinc could not suppress acetylsalicylic acid -induced apoptosis at its late phase. We conclude that polaprezinc-increased Heat shock protein 70 expression might be an important mechanism by which polaprezinc suppresses acetylsalicylic acid-induced small intestinal apoptosis, a hallmark of acetylsalicylic acid-induced enteropathy.
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spelling pubmed-32080132011-11-29 Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells Qin, Ying Naito, Yuji Handa, Osamu Hayashi, Natsuko Kuki, Aiko Mizushima, Katsura Omatsu, Tatsushi Tanimura, Yuko Morita, Mayuko Adachi, Satoko Fukui, Akifumi Hirata, Ikuhiro Kishimoto, Etsuko Nishikawa, Taichiro Uchiyama, Kazuhiko Ishikawa, Takeshi Takagi, Tomohisa Yagi, Nobuaki Kokura, Satoshi Yoshikawa, Toshikazu J Clin Biochem Nutr Original Article Protection of the small intestine from mucosal injury induced by nonsteroidal anti-inflammatory drugs including acetylsalicylic acid is a critical issue in the field of gastroenterology. Polaprezinc an anti-ulcer drug, consisting of zinc and L-carnosine, provides gastric mucosal protection against various irritants. In this study, we investigated the protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of the RIE1 rat intestinal epithelial cell line. Confluent rat intestinal epithelial cells were incubated with 70 µM polaprezinc for 24 h, and then stimulated with or without 15 mM acetylsalicylic acid for a further 15 h. Subsequent cellular viability was quantified by fluorometric assay based on cell lysis and staining. Acetylsalicylic acid-induced cell death was also qualified by fluorescent microscopy of Hoechst33342 and propidium iodide. Heat shock proteins 70 protein expression after adding polaprezinc or acetylsalicylic acid was assessed by western blotting. To investigate the role of Heat shock protein 70, Heat shock protein 70-specific small interfering RNA was applied. Cell viability was quantified by fluorometric assay based on cell lysis and staining and apoptosis was analyzed by fluorescence-activated cell sorting. We found that acetylsalicylic acid significantly induced apoptosis of rat intestinal epithelial cells in a dose- and time-dependent manner. Polaprezinc significantly suppressed acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells at its late phase. At the same time, polaprezinc increased Heat shock protein 70 expressions of rat intestinal epithelial cells in a time-dependent manner. However, in Heat shock protein 70-silenced rat intestinal epithelial cells, polaprezinc could not suppress acetylsalicylic acid -induced apoptosis at its late phase. We conclude that polaprezinc-increased Heat shock protein 70 expression might be an important mechanism by which polaprezinc suppresses acetylsalicylic acid-induced small intestinal apoptosis, a hallmark of acetylsalicylic acid-induced enteropathy. the Society for Free Radical Research Japan 2011-11 2011-06-17 /pmc/articles/PMC3208013/ /pubmed/22128216 http://dx.doi.org/10.3164/jcbn.11-26 Text en Copyright © 2011 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Qin, Ying
Naito, Yuji
Handa, Osamu
Hayashi, Natsuko
Kuki, Aiko
Mizushima, Katsura
Omatsu, Tatsushi
Tanimura, Yuko
Morita, Mayuko
Adachi, Satoko
Fukui, Akifumi
Hirata, Ikuhiro
Kishimoto, Etsuko
Nishikawa, Taichiro
Uchiyama, Kazuhiko
Ishikawa, Takeshi
Takagi, Tomohisa
Yagi, Nobuaki
Kokura, Satoshi
Yoshikawa, Toshikazu
Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title_full Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title_fullStr Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title_full_unstemmed Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title_short Heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
title_sort heat shock protein 70-dependent protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3208013/
https://www.ncbi.nlm.nih.gov/pubmed/22128216
http://dx.doi.org/10.3164/jcbn.11-26
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