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Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage
The bi-directional communication between the oocyte and the surrounding cumulus cells (CCs) is crucial for the acquisition of oocyte competence. We investigated the transcriptomic profile of human CCs isolated from mature and immature oocytes under stimulated cycle. We used human Genome U133 Plus 2....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210145/ https://www.ncbi.nlm.nih.gov/pubmed/22087263 http://dx.doi.org/10.1371/journal.pone.0027179 |
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author | Ouandaogo, Zamalou Gisèle Haouzi, Delphine Assou, Said Dechaud, Hervé Kadoch, Issac Jacques De Vos, John Hamamah, Samir |
author_facet | Ouandaogo, Zamalou Gisèle Haouzi, Delphine Assou, Said Dechaud, Hervé Kadoch, Issac Jacques De Vos, John Hamamah, Samir |
author_sort | Ouandaogo, Zamalou Gisèle |
collection | PubMed |
description | The bi-directional communication between the oocyte and the surrounding cumulus cells (CCs) is crucial for the acquisition of oocyte competence. We investigated the transcriptomic profile of human CCs isolated from mature and immature oocytes under stimulated cycle. We used human Genome U133 Plus 2.0 microarrays to perform an extensive analysis of the genes expressed in human CCs obtained from patients undergoing intra-cytoplasmic sperm injection. CC samples were isolated from oocyte at germinal vesicle, stage metaphase I and stage metaphase II. For microarray analysis, we used eight chips for each CC category. Significance analysis of microarray multiclass was used to analyze the microarray data. Validation was performed by RT-qPCR using an independent cohort of CC samples. We identified differentially over-expressed genes between the three CC categories. This study revealed a specific signature of gene expression in CCs issued from MII oocyte compared with germinal vesicle and metaphase I. The CC gene expression profile, which is specific of MII mature oocyte, can be useful as predictors of oocyte quality. |
format | Online Article Text |
id | pubmed-3210145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32101452011-11-15 Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage Ouandaogo, Zamalou Gisèle Haouzi, Delphine Assou, Said Dechaud, Hervé Kadoch, Issac Jacques De Vos, John Hamamah, Samir PLoS One Research Article The bi-directional communication between the oocyte and the surrounding cumulus cells (CCs) is crucial for the acquisition of oocyte competence. We investigated the transcriptomic profile of human CCs isolated from mature and immature oocytes under stimulated cycle. We used human Genome U133 Plus 2.0 microarrays to perform an extensive analysis of the genes expressed in human CCs obtained from patients undergoing intra-cytoplasmic sperm injection. CC samples were isolated from oocyte at germinal vesicle, stage metaphase I and stage metaphase II. For microarray analysis, we used eight chips for each CC category. Significance analysis of microarray multiclass was used to analyze the microarray data. Validation was performed by RT-qPCR using an independent cohort of CC samples. We identified differentially over-expressed genes between the three CC categories. This study revealed a specific signature of gene expression in CCs issued from MII oocyte compared with germinal vesicle and metaphase I. The CC gene expression profile, which is specific of MII mature oocyte, can be useful as predictors of oocyte quality. Public Library of Science 2011-11-07 /pmc/articles/PMC3210145/ /pubmed/22087263 http://dx.doi.org/10.1371/journal.pone.0027179 Text en Ouandaogo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ouandaogo, Zamalou Gisèle Haouzi, Delphine Assou, Said Dechaud, Hervé Kadoch, Issac Jacques De Vos, John Hamamah, Samir Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title | Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title_full | Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title_fullStr | Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title_full_unstemmed | Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title_short | Human Cumulus Cells Molecular Signature in Relation to Oocyte Nuclear Maturity Stage |
title_sort | human cumulus cells molecular signature in relation to oocyte nuclear maturity stage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210145/ https://www.ncbi.nlm.nih.gov/pubmed/22087263 http://dx.doi.org/10.1371/journal.pone.0027179 |
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