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Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in associa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japan Society of Histochemistry and Cytochemistry
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210425/ https://www.ncbi.nlm.nih.gov/pubmed/22096260 http://dx.doi.org/10.1267/ahc.10039 |
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author | Yahiro, Junko Inai, Tetsuichiro Tsutsui, Akihito Sato, Atsuko Nagato, Toshikazu Taniguchi, Kunihisa Tsuruga, Eichi Sawa, Yoshihiko |
author_facet | Yahiro, Junko Inai, Tetsuichiro Tsutsui, Akihito Sato, Atsuko Nagato, Toshikazu Taniguchi, Kunihisa Tsuruga, Eichi Sawa, Yoshihiko |
author_sort | Yahiro, Junko |
collection | PubMed |
description | The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner’s glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner’s glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner’s glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner’s acini compared to parotid acini, whereas von Ebner’s ducts may secrete significantly more amylase to modify saliva than parotid ducts. |
format | Online Article Text |
id | pubmed-3210425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Japan Society of Histochemistry and Cytochemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-32104252011-11-17 Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy Yahiro, Junko Inai, Tetsuichiro Tsutsui, Akihito Sato, Atsuko Nagato, Toshikazu Taniguchi, Kunihisa Tsuruga, Eichi Sawa, Yoshihiko Acta Histochem Cytochem Regular Article The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner’s glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner’s glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner’s glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner’s acini compared to parotid acini, whereas von Ebner’s ducts may secrete significantly more amylase to modify saliva than parotid ducts. Japan Society of Histochemistry and Cytochemistry 2011-10-26 2011-08-10 /pmc/articles/PMC3210425/ /pubmed/22096260 http://dx.doi.org/10.1267/ahc.10039 Text en © 2011 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Yahiro, Junko Inai, Tetsuichiro Tsutsui, Akihito Sato, Atsuko Nagato, Toshikazu Taniguchi, Kunihisa Tsuruga, Eichi Sawa, Yoshihiko Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title | Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title_full | Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title_fullStr | Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title_full_unstemmed | Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title_short | Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy |
title_sort | immunohistochemical and immunocytochemical localization of amylase in rat parotid glands and von ebner’s glands by ion etching-immunoscanning electron microscopy |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210425/ https://www.ncbi.nlm.nih.gov/pubmed/22096260 http://dx.doi.org/10.1267/ahc.10039 |
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