Cargando…

Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy

The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in associa...

Descripción completa

Detalles Bibliográficos
Autores principales: Yahiro, Junko, Inai, Tetsuichiro, Tsutsui, Akihito, Sato, Atsuko, Nagato, Toshikazu, Taniguchi, Kunihisa, Tsuruga, Eichi, Sawa, Yoshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210425/
https://www.ncbi.nlm.nih.gov/pubmed/22096260
http://dx.doi.org/10.1267/ahc.10039
_version_ 1782215732015136768
author Yahiro, Junko
Inai, Tetsuichiro
Tsutsui, Akihito
Sato, Atsuko
Nagato, Toshikazu
Taniguchi, Kunihisa
Tsuruga, Eichi
Sawa, Yoshihiko
author_facet Yahiro, Junko
Inai, Tetsuichiro
Tsutsui, Akihito
Sato, Atsuko
Nagato, Toshikazu
Taniguchi, Kunihisa
Tsuruga, Eichi
Sawa, Yoshihiko
author_sort Yahiro, Junko
collection PubMed
description The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner’s glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner’s glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner’s glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner’s acini compared to parotid acini, whereas von Ebner’s ducts may secrete significantly more amylase to modify saliva than parotid ducts.
format Online
Article
Text
id pubmed-3210425
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Japan Society of Histochemistry and Cytochemistry
record_format MEDLINE/PubMed
spelling pubmed-32104252011-11-17 Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy Yahiro, Junko Inai, Tetsuichiro Tsutsui, Akihito Sato, Atsuko Nagato, Toshikazu Taniguchi, Kunihisa Tsuruga, Eichi Sawa, Yoshihiko Acta Histochem Cytochem Regular Article The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner’s glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner’s glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner’s glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner’s acini compared to parotid acini, whereas von Ebner’s ducts may secrete significantly more amylase to modify saliva than parotid ducts. Japan Society of Histochemistry and Cytochemistry 2011-10-26 2011-08-10 /pmc/articles/PMC3210425/ /pubmed/22096260 http://dx.doi.org/10.1267/ahc.10039 Text en © 2011 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Yahiro, Junko
Inai, Tetsuichiro
Tsutsui, Akihito
Sato, Atsuko
Nagato, Toshikazu
Taniguchi, Kunihisa
Tsuruga, Eichi
Sawa, Yoshihiko
Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title_full Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title_fullStr Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title_full_unstemmed Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title_short Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner’s Glands by Ion Etching-Immunoscanning Electron Microscopy
title_sort immunohistochemical and immunocytochemical localization of amylase in rat parotid glands and von ebner’s glands by ion etching-immunoscanning electron microscopy
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210425/
https://www.ncbi.nlm.nih.gov/pubmed/22096260
http://dx.doi.org/10.1267/ahc.10039
work_keys_str_mv AT yahirojunko immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT inaitetsuichiro immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT tsutsuiakihito immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT satoatsuko immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT nagatotoshikazu immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT taniguchikunihisa immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT tsurugaeichi immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy
AT sawayoshihiko immunohistochemicalandimmunocytochemicallocalizationofamylaseinratparotidglandsandvonebnersglandsbyionetchingimmunoscanningelectronmicroscopy