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PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to...

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Autores principales: Moon, Joung-Ho, Cho, Shin-Hyeong, Yu, Jae-Ran, Lee, Won-Ja, Cheun, Hyeng-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210846/
https://www.ncbi.nlm.nih.gov/pubmed/22072829
http://dx.doi.org/10.3347/kjp.2011.49.3.281
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author Moon, Joung-Ho
Cho, Shin-Hyeong
Yu, Jae-Ran
Lee, Won-Ja
Cheun, Hyeng-Il
author_facet Moon, Joung-Ho
Cho, Shin-Hyeong
Yu, Jae-Ran
Lee, Won-Ja
Cheun, Hyeng-Il
author_sort Moon, Joung-Ho
collection PubMed
description Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.
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spelling pubmed-32108462011-11-09 PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples Moon, Joung-Ho Cho, Shin-Hyeong Yu, Jae-Ran Lee, Won-Ja Cheun, Hyeng-Il Korean J Parasitol Brief Communication Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples. The Korean Society for Parasitology 2011-09 2011-09-30 /pmc/articles/PMC3210846/ /pubmed/22072829 http://dx.doi.org/10.3347/kjp.2011.49.3.281 Text en © 2011, Korean Society for Parasitology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Moon, Joung-Ho
Cho, Shin-Hyeong
Yu, Jae-Ran
Lee, Won-Ja
Cheun, Hyeng-Il
PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title_full PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title_fullStr PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title_full_unstemmed PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title_short PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
title_sort pcr diagnosis of entamoeba histolytica cysts in stool samples
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210846/
https://www.ncbi.nlm.nih.gov/pubmed/22072829
http://dx.doi.org/10.3347/kjp.2011.49.3.281
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