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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells

Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity...

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Autores principales: Pi, Woojin, Ryu, Jae-Sook, Roh, Jaesook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210849/
https://www.ncbi.nlm.nih.gov/pubmed/22072832
http://dx.doi.org/10.3347/kjp.2011.49.3.295
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author Pi, Woojin
Ryu, Jae-Sook
Roh, Jaesook
author_facet Pi, Woojin
Ryu, Jae-Sook
Roh, Jaesook
author_sort Pi, Woojin
collection PubMed
description Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.
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spelling pubmed-32108492011-11-09 Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells Pi, Woojin Ryu, Jae-Sook Roh, Jaesook Korean J Parasitol Brief Communication Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells. The Korean Society for Parasitology 2011-09 2011-09-30 /pmc/articles/PMC3210849/ /pubmed/22072832 http://dx.doi.org/10.3347/kjp.2011.49.3.295 Text en © 2011, Korean Society for Parasitology http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Pi, Woojin
Ryu, Jae-Sook
Roh, Jaesook
Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title_full Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title_fullStr Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title_full_unstemmed Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title_short Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
title_sort lactobacillus acidophilus contributes to a healthy environment for vaginal epithelial cells
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210849/
https://www.ncbi.nlm.nih.gov/pubmed/22072832
http://dx.doi.org/10.3347/kjp.2011.49.3.295
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