Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects

Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange hi...

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Detalles Bibliográficos
Autores principales: Khunsap, Suchitra, Pakmanee, Narumol, Khow, Orawan, Chanhome, Lawan, Sitprija, Visith, Suntravat, Montamas, Lucena, Sara E, Perez, John C, Sánchez, Elda E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Library Publishing Media 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210966/
https://www.ncbi.nlm.nih.gov/pubmed/22091349
Descripción
Sumario:Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA(2) was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA(2)s of viperid snake venoms. The Drs-PLA(2) had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC(50) of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC(50) of 25.6nM. In addition, the Drs-PLA(2) inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%.

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