Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects

Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange hi...

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Autores principales: Khunsap, Suchitra, Pakmanee, Narumol, Khow, Orawan, Chanhome, Lawan, Sitprija, Visith, Suntravat, Montamas, Lucena, Sara E, Perez, John C, Sánchez, Elda E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Library Publishing Media 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210966/
https://www.ncbi.nlm.nih.gov/pubmed/22091349
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author Khunsap, Suchitra
Pakmanee, Narumol
Khow, Orawan
Chanhome, Lawan
Sitprija, Visith
Suntravat, Montamas
Lucena, Sara E
Perez, John C
Sánchez, Elda E
author_facet Khunsap, Suchitra
Pakmanee, Narumol
Khow, Orawan
Chanhome, Lawan
Sitprija, Visith
Suntravat, Montamas
Lucena, Sara E
Perez, John C
Sánchez, Elda E
author_sort Khunsap, Suchitra
collection PubMed
description Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA(2) was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA(2)s of viperid snake venoms. The Drs-PLA(2) had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC(50) of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC(50) of 25.6nM. In addition, the Drs-PLA(2) inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%.
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spelling pubmed-32109662011-11-16 Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects Khunsap, Suchitra Pakmanee, Narumol Khow, Orawan Chanhome, Lawan Sitprija, Visith Suntravat, Montamas Lucena, Sara E Perez, John C Sánchez, Elda E J Venom Res Research Article Venom phospholipases A2 (PLA(2)) are associated with neurotoxic, myotoxic, cardiotoxic, platelet aggregation, and edema activities. A PLA(2) (Drs-PLA(2)) was purified from Daboia russelii siamensis venom by a two-step purification procedure consisting of size-exclusion, followed by anion exchange high performance liquid chromatography (HPLC). The molecular weight of the Drs-PLA(2) was 13,679Da, which was determined by MALDI-TOF mass spectrometry. Its N-terminal amino acid sequence was homologous to basic PLA(2)s of viperid snake venoms. The Drs-PLA(2) had indirect hemolytic and anticoagulant activities, cytotoxic activity with a CC(50) of 65.8nM, and inhibited SK-MEL-28 cell migration with an IC(50) of 25.6nM. In addition, the Drs-PLA(2) inhibited the colonization of B16F10 cells in lungs of BALB/c mice by ∼65%. Library Publishing Media 2011-10-22 /pmc/articles/PMC3210966/ /pubmed/22091349 Text en © Copyright The Author(s) http://creativecommons.org/licenses/by-nc/2.5 Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.
spellingShingle Research Article
Khunsap, Suchitra
Pakmanee, Narumol
Khow, Orawan
Chanhome, Lawan
Sitprija, Visith
Suntravat, Montamas
Lucena, Sara E
Perez, John C
Sánchez, Elda E
Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title_full Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title_fullStr Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title_full_unstemmed Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title_short Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects
title_sort purification of a phospholipase a(2) from daboia russelii siamensis venom with anticancer effects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210966/
https://www.ncbi.nlm.nih.gov/pubmed/22091349
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