A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)

A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH(2)Cl(2), EtOAc and n-BuOH to afford four fractions: CH(2)Cl(2)-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activit...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Yang, Cheng, Xuzhen, Wang, Lixia, Wang, Suhua, Ren, Guixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210989/
https://www.ncbi.nlm.nih.gov/pubmed/22072898
http://dx.doi.org/10.3390/ijms12106445
_version_ 1782215784751169536
author Yao, Yang
Cheng, Xuzhen
Wang, Lixia
Wang, Suhua
Ren, Guixing
author_facet Yao, Yang
Cheng, Xuzhen
Wang, Lixia
Wang, Suhua
Ren, Guixing
author_sort Yao, Yang
collection PubMed
description A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH(2)Cl(2), EtOAc and n-BuOH to afford four fractions: CH(2)Cl(2)-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC(50) values of 0.4 mg·mL(−1) and 4.8 mg·mL(−1), respectively. This is the first study of the active compositions of azuki beans against α-glucosidase.
format Online
Article
Text
id pubmed-3210989
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-32109892011-11-09 A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis) Yao, Yang Cheng, Xuzhen Wang, Lixia Wang, Suhua Ren, Guixing Int J Mol Sci Article A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH(2)Cl(2), EtOAc and n-BuOH to afford four fractions: CH(2)Cl(2)-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC(50) values of 0.4 mg·mL(−1) and 4.8 mg·mL(−1), respectively. This is the first study of the active compositions of azuki beans against α-glucosidase. Molecular Diversity Preservation International (MDPI) 2011-09-28 /pmc/articles/PMC3210989/ /pubmed/22072898 http://dx.doi.org/10.3390/ijms12106445 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yao, Yang
Cheng, Xuzhen
Wang, Lixia
Wang, Suhua
Ren, Guixing
A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title_full A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title_fullStr A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title_full_unstemmed A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title_short A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)
title_sort determination of potential α-glucosidase inhibitors from azuki beans (vigna angularis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210989/
https://www.ncbi.nlm.nih.gov/pubmed/22072898
http://dx.doi.org/10.3390/ijms12106445
work_keys_str_mv AT yaoyang adeterminationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT chengxuzhen adeterminationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT wanglixia adeterminationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT wangsuhua adeterminationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT renguixing adeterminationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT yaoyang determinationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT chengxuzhen determinationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT wanglixia determinationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT wangsuhua determinationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis
AT renguixing determinationofpotentialaglucosidaseinhibitorsfromazukibeansvignaangularis

Ejemplares similares