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The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction

PURPOSE: Our purpose was to conduct a screening test for urethritis or cervicitis as a sexually transmitted disease (STD) by using multiplex polymerase chain reaction(PCR) and to determine the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum,...

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Autores principales: Kim, Suk-Ju, Lee, Dong Sup, Lee, Seung-Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Urological Association 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212666/
https://www.ncbi.nlm.nih.gov/pubmed/22087366
http://dx.doi.org/10.4111/kju.2011.52.10.703
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author Kim, Suk-Ju
Lee, Dong Sup
Lee, Seung-Ju
author_facet Kim, Suk-Ju
Lee, Dong Sup
Lee, Seung-Ju
author_sort Kim, Suk-Ju
collection PubMed
description PURPOSE: Our purpose was to conduct a screening test for urethritis or cervicitis as a sexually transmitted disease (STD) by using multiplex polymerase chain reaction(PCR) and to determine the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis in asymptomatic people. MATERIALS AND METHODS: From July 2010 to December 2010, 709 persons who came to the hospital for a general checkup were tested. Multiplex PCR assays were done with first voided urine samples or endocervical swabs by use of the Seeplex® STD6 ACE Detection kit. RESULTS: The mean age in this study was 45.4±8.1 years. Among the 709 persons, 229 (32.3%) had a positive result for at least one microorganism, 48 (6.8%) had two different species, 6 (0.8%) had three different species, and 1 person had four different species. The overall prevalence of asymptomatic STDs such as urethritis or cervicitis was 7.1% (50/709). The prevalence rates of chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis infection in asymptomatic persons were 5.6% (40/709), 0.4% (3/709), 0.3% (2/709), 22.1% (157/709), 11.6% (82/709), and 1.1% (8/709), respectively. CONCLUSIONS: With only a single sample, we could identify the prevalence rates of six microorganisms and the overall proportion of urethritis or cervicitis in asymptomatic people. This proportion cannot be neglected; therefore, screening tests for sexually transmitted diseases such as urethritis or ervicitis should be recommended to asymptomatic people.
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spelling pubmed-32126662011-11-15 The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction Kim, Suk-Ju Lee, Dong Sup Lee, Seung-Ju Korean J Urol Original Article PURPOSE: Our purpose was to conduct a screening test for urethritis or cervicitis as a sexually transmitted disease (STD) by using multiplex polymerase chain reaction(PCR) and to determine the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis in asymptomatic people. MATERIALS AND METHODS: From July 2010 to December 2010, 709 persons who came to the hospital for a general checkup were tested. Multiplex PCR assays were done with first voided urine samples or endocervical swabs by use of the Seeplex® STD6 ACE Detection kit. RESULTS: The mean age in this study was 45.4±8.1 years. Among the 709 persons, 229 (32.3%) had a positive result for at least one microorganism, 48 (6.8%) had two different species, 6 (0.8%) had three different species, and 1 person had four different species. The overall prevalence of asymptomatic STDs such as urethritis or cervicitis was 7.1% (50/709). The prevalence rates of chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis infection in asymptomatic persons were 5.6% (40/709), 0.4% (3/709), 0.3% (2/709), 22.1% (157/709), 11.6% (82/709), and 1.1% (8/709), respectively. CONCLUSIONS: With only a single sample, we could identify the prevalence rates of six microorganisms and the overall proportion of urethritis or cervicitis in asymptomatic people. This proportion cannot be neglected; therefore, screening tests for sexually transmitted diseases such as urethritis or ervicitis should be recommended to asymptomatic people. The Korean Urological Association 2011-10 2011-10-19 /pmc/articles/PMC3212666/ /pubmed/22087366 http://dx.doi.org/10.4111/kju.2011.52.10.703 Text en © The Korean Urological Association, 2011 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Suk-Ju
Lee, Dong Sup
Lee, Seung-Ju
The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title_full The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title_fullStr The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title_full_unstemmed The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title_short The Prevalence and Clinical Significance of Urethritis and Cervicitis in Asymptomatic People by Use of Multiplex Polymerase Chain Reaction
title_sort prevalence and clinical significance of urethritis and cervicitis in asymptomatic people by use of multiplex polymerase chain reaction
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212666/
https://www.ncbi.nlm.nih.gov/pubmed/22087366
http://dx.doi.org/10.4111/kju.2011.52.10.703
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