Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries

To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screenin...

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Detalles Bibliográficos
Autores principales: Okabe, Yoshihiro, Asamizu, Erika, Saito, Takeshi, Matsukura, Chiaki, Ariizumi, Tohru, Brès, Cécile, Rothan, Christophe, Mizoguchi, Tsuyoshi, Ezura, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Regular Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212723/
https://www.ncbi.nlm.nih.gov/pubmed/21965606
http://dx.doi.org/10.1093/pcp/pcr134

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