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Determinants of nucleosome organization in primary human cells
Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodeling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other func...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212987/ https://www.ncbi.nlm.nih.gov/pubmed/21602827 http://dx.doi.org/10.1038/nature10002 |
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author | Valouev, Anton Johnson, Steven M. Boyd, Scott D. Smith, Cheryl L. Fire, Andrew Z. Sidow, Arend |
author_facet | Valouev, Anton Johnson, Steven M. Boyd, Scott D. Smith, Cheryl L. Fire, Andrew Z. Sidow, Arend |
author_sort | Valouev, Anton |
collection | PubMed |
description | Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodeling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome(1). Analyses of chromatin landscape have uncovered a variety of mechanisms, including DNA sequence preferences, that can influence nucleosome positions(2–4). To identify major determinants of nucleosome organization in the human genome, we utilized deep sequencing to map nucleosome positions in three primary human cell types and in vitro. A majority of the genome exhibited substantial flexibility of nucleosome positions while a small fraction showed reproducibly positioned nucleosomes. Certain sites that position in vitro can anchor the formation of nucleosomal arrays that have cell type-specific spacing in vivo. Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells. |
format | Online Article Text |
id | pubmed-3212987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-32129872011-12-23 Determinants of nucleosome organization in primary human cells Valouev, Anton Johnson, Steven M. Boyd, Scott D. Smith, Cheryl L. Fire, Andrew Z. Sidow, Arend Nature Article Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodeling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome(1). Analyses of chromatin landscape have uncovered a variety of mechanisms, including DNA sequence preferences, that can influence nucleosome positions(2–4). To identify major determinants of nucleosome organization in the human genome, we utilized deep sequencing to map nucleosome positions in three primary human cell types and in vitro. A majority of the genome exhibited substantial flexibility of nucleosome positions while a small fraction showed reproducibly positioned nucleosomes. Certain sites that position in vitro can anchor the formation of nucleosomal arrays that have cell type-specific spacing in vivo. Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells. 2011-05-22 /pmc/articles/PMC3212987/ /pubmed/21602827 http://dx.doi.org/10.1038/nature10002 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Valouev, Anton Johnson, Steven M. Boyd, Scott D. Smith, Cheryl L. Fire, Andrew Z. Sidow, Arend Determinants of nucleosome organization in primary human cells |
title | Determinants of nucleosome organization in primary human cells |
title_full | Determinants of nucleosome organization in primary human cells |
title_fullStr | Determinants of nucleosome organization in primary human cells |
title_full_unstemmed | Determinants of nucleosome organization in primary human cells |
title_short | Determinants of nucleosome organization in primary human cells |
title_sort | determinants of nucleosome organization in primary human cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212987/ https://www.ncbi.nlm.nih.gov/pubmed/21602827 http://dx.doi.org/10.1038/nature10002 |
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