Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model

Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities...

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Detalles Bibliográficos
Autores principales: Swanger, Sharon A, Yao, Xiaodi, Gross, Christina, Bassell, Gary J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213078/
https://www.ncbi.nlm.nih.gov/pubmed/21982080
http://dx.doi.org/10.1186/1756-6606-4-38
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author Swanger, Sharon A
Yao, Xiaodi
Gross, Christina
Bassell, Gary J
author_facet Swanger, Sharon A
Yao, Xiaodi
Gross, Christina
Bassell, Gary J
author_sort Swanger, Sharon A
collection PubMed
description Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities in a disease model. We anticipate that this approach will advance studies of synapse structure and function in brain development, plasticity, and disease.
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spelling pubmed-32130782011-11-14 Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model Swanger, Sharon A Yao, Xiaodi Gross, Christina Bassell, Gary J Mol Brain Methodology Uncovering the mechanisms that regulate dendritic spine morphology has been limited, in part, by the lack of efficient and unbiased methods for analyzing spines. Here, we describe an automated 3D spine morphometry method and its application to spine remodeling in live neurons and spine abnormalities in a disease model. We anticipate that this approach will advance studies of synapse structure and function in brain development, plasticity, and disease. BioMed Central 2011-10-07 /pmc/articles/PMC3213078/ /pubmed/21982080 http://dx.doi.org/10.1186/1756-6606-4-38 Text en Copyright ©2011 Swanger et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Swanger, Sharon A
Yao, Xiaodi
Gross, Christina
Bassell, Gary J
Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title_full Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title_fullStr Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title_full_unstemmed Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title_short Automated 4D analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
title_sort automated 4d analysis of dendritic spine morphology: applications to stimulus-induced spine remodeling and pharmacological rescue in a disease model
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213078/
https://www.ncbi.nlm.nih.gov/pubmed/21982080
http://dx.doi.org/10.1186/1756-6606-4-38
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