Cytolytic replication of echoviruses in colon cancer cell lines

BACKGROUND: Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a ca...

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Autores principales: Israelsson, Stina, Jonsson, Nina, Gullberg, Maria, Lindberg, A Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213228/
https://www.ncbi.nlm.nih.gov/pubmed/21999585
http://dx.doi.org/10.1186/1743-422X-8-473
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author Israelsson, Stina
Jonsson, Nina
Gullberg, Maria
Lindberg, A Michael
author_facet Israelsson, Stina
Jonsson, Nina
Gullberg, Maria
Lindberg, A Michael
author_sort Israelsson, Stina
collection PubMed
description BACKGROUND: Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a cancer is cured by viral infection, is a promising field for finding new and improved treatments. We have investigated the oncolytic potential of several low-pathogenic echoviruses with rare clinical occurrence. Echoviruses are members of the enterovirus genus within the family Picornaviridae. METHODS: Six colon cancer cell lines (CaCo-2, HT29, LoVo, SW480, SW620 and T84) were infected by the human enterovirus B species echovirus 12, 15, 17, 26 and 29, and cytopathic effects as well as viral replication efficacy were investigated. Infectivity was also tested in spheroids grown from HT29 cells. RESULTS: Echovirus 12, 17, 26 and 29 replicated efficiently in almost all cell lines and were considered highly cytolytic. The infectivity of these four viruses was further evaluated in artificial tumors (spheroids), where it was found that echovirus 12, 17 and 26 easily infected the spheroids. CONCLUSIONS: We have found that echovirus 12, 17 and 26 have potential as oncolytic agents against colon cancer, by comparing the cytolytic capacity of five low-pathogenic echoviruses in six colon cancer cell lines and in artificial tumors.
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spelling pubmed-32132282011-11-11 Cytolytic replication of echoviruses in colon cancer cell lines Israelsson, Stina Jonsson, Nina Gullberg, Maria Lindberg, A Michael Virol J Research BACKGROUND: Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a cancer is cured by viral infection, is a promising field for finding new and improved treatments. We have investigated the oncolytic potential of several low-pathogenic echoviruses with rare clinical occurrence. Echoviruses are members of the enterovirus genus within the family Picornaviridae. METHODS: Six colon cancer cell lines (CaCo-2, HT29, LoVo, SW480, SW620 and T84) were infected by the human enterovirus B species echovirus 12, 15, 17, 26 and 29, and cytopathic effects as well as viral replication efficacy were investigated. Infectivity was also tested in spheroids grown from HT29 cells. RESULTS: Echovirus 12, 17, 26 and 29 replicated efficiently in almost all cell lines and were considered highly cytolytic. The infectivity of these four viruses was further evaluated in artificial tumors (spheroids), where it was found that echovirus 12, 17 and 26 easily infected the spheroids. CONCLUSIONS: We have found that echovirus 12, 17 and 26 have potential as oncolytic agents against colon cancer, by comparing the cytolytic capacity of five low-pathogenic echoviruses in six colon cancer cell lines and in artificial tumors. BioMed Central 2011-10-14 /pmc/articles/PMC3213228/ /pubmed/21999585 http://dx.doi.org/10.1186/1743-422X-8-473 Text en Copyright ©2011 Israelsson et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Israelsson, Stina
Jonsson, Nina
Gullberg, Maria
Lindberg, A Michael
Cytolytic replication of echoviruses in colon cancer cell lines
title Cytolytic replication of echoviruses in colon cancer cell lines
title_full Cytolytic replication of echoviruses in colon cancer cell lines
title_fullStr Cytolytic replication of echoviruses in colon cancer cell lines
title_full_unstemmed Cytolytic replication of echoviruses in colon cancer cell lines
title_short Cytolytic replication of echoviruses in colon cancer cell lines
title_sort cytolytic replication of echoviruses in colon cancer cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213228/
https://www.ncbi.nlm.nih.gov/pubmed/21999585
http://dx.doi.org/10.1186/1743-422X-8-473
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