Cargando…

Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity

Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-...

Descripción completa

Detalles Bibliográficos
Autores principales: Annamalai, Neelamegam, Veeramuthu Rajeswari, Mayavan, Vijayalakshmi, Shanmugam, Balasubramanian, Thangavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213332/
https://www.ncbi.nlm.nih.gov/pubmed/22131949
http://dx.doi.org/10.1007/s13213-011-0198-5
_version_ 1782216115201507328
author Annamalai, Neelamegam
Veeramuthu Rajeswari, Mayavan
Vijayalakshmi, Shanmugam
Balasubramanian, Thangavel
author_facet Annamalai, Neelamegam
Veeramuthu Rajeswari, Mayavan
Vijayalakshmi, Shanmugam
Balasubramanian, Thangavel
author_sort Annamalai, Neelamegam
collection PubMed
description Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K(2)HPO(4), and 0.05% MgSO(4)·7H(2)O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5–12, and 40–80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine.
format Online
Article
Text
id pubmed-3213332
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-32133322011-11-28 Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity Annamalai, Neelamegam Veeramuthu Rajeswari, Mayavan Vijayalakshmi, Shanmugam Balasubramanian, Thangavel Ann Microbiol Original Article Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K(2)HPO(4), and 0.05% MgSO(4)·7H(2)O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5–12, and 40–80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine. Springer-Verlag 2011-01-22 2011 /pmc/articles/PMC3213332/ /pubmed/22131949 http://dx.doi.org/10.1007/s13213-011-0198-5 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Annamalai, Neelamegam
Veeramuthu Rajeswari, Mayavan
Vijayalakshmi, Shanmugam
Balasubramanian, Thangavel
Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title_full Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title_fullStr Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title_full_unstemmed Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title_short Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
title_sort purification and characterization of chitinase from alcaligenes faecalis au02 by utilizing marine wastes and its antioxidant activity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3213332/
https://www.ncbi.nlm.nih.gov/pubmed/22131949
http://dx.doi.org/10.1007/s13213-011-0198-5
work_keys_str_mv AT annamalaineelamegam purificationandcharacterizationofchitinasefromalcaligenesfaecalisau02byutilizingmarinewastesanditsantioxidantactivity
AT veeramuthurajeswarimayavan purificationandcharacterizationofchitinasefromalcaligenesfaecalisau02byutilizingmarinewastesanditsantioxidantactivity
AT vijayalakshmishanmugam purificationandcharacterizationofchitinasefromalcaligenesfaecalisau02byutilizingmarinewastesanditsantioxidantactivity
AT balasubramanianthangavel purificationandcharacterizationofchitinasefromalcaligenesfaecalisau02byutilizingmarinewastesanditsantioxidantactivity