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Construction and Characterization of Single-Chain Variable Fragment Antibody Library Derived from Germline Rearranged Immunoglobulin Variable Genes

Antibody repertoires for library construction are conventionally harvested from mRNAs of immune cells. To examine whether germline rearranged immunoglobulin (Ig) variable region genes could be used as source of antibody repertoire, an immunized phage-displayed scFv library was prepared using splenoc...

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Detalles Bibliográficos
Autores principales:	Cheng, Man, Chan, Shirley Y. W., Zhao, Qi, Chan, Elaine Y. M., Au, Shannon W. N., Lee, Susanna S. T., Cheung, Wing-Tai
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214059/ https://www.ncbi.nlm.nih.gov/pubmed/22096568 http://dx.doi.org/10.1371/journal.pone.0027406

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