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New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall

β-glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-glucans provide structural integrity to the fungal cell wall. The nature of the (1–6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to el...

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Autores principales: Lowman, Douglas W., West, Lara J., Bearden, Daniel W., Wempe, Michael F., Power, Trevor D., Ensley, Harry E., Haynes, Ken, Williams, David L., Kruppa, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214063/
https://www.ncbi.nlm.nih.gov/pubmed/22096604
http://dx.doi.org/10.1371/journal.pone.0027614
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author Lowman, Douglas W.
West, Lara J.
Bearden, Daniel W.
Wempe, Michael F.
Power, Trevor D.
Ensley, Harry E.
Haynes, Ken
Williams, David L.
Kruppa, Michael D.
author_facet Lowman, Douglas W.
West, Lara J.
Bearden, Daniel W.
Wempe, Michael F.
Power, Trevor D.
Ensley, Harry E.
Haynes, Ken
Williams, David L.
Kruppa, Michael D.
author_sort Lowman, Douglas W.
collection PubMed
description β-glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-glucans provide structural integrity to the fungal cell wall. The nature of the (1–6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity.
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spelling pubmed-32140632011-11-17 New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall Lowman, Douglas W. West, Lara J. Bearden, Daniel W. Wempe, Michael F. Power, Trevor D. Ensley, Harry E. Haynes, Ken Williams, David L. Kruppa, Michael D. PLoS One Research Article β-glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and a major component of fungal cell walls. β-glucans provide structural integrity to the fungal cell wall. The nature of the (1–6)-β-linked side chain structure of fungal (1→3,1→6)-β-D-glucans has been very difficult to elucidate. Herein, we report the first detailed structural characterization of the (1→6)-β-linked side chains of Candida glabrata using high-field NMR. The (1→6)-β-linked side chains have an average length of 4 to 5 repeat units spaced every 21 repeat units along the (1→3)-linked polymer backbone. Computer modeling suggests that the side chains have a bent curve structure that allows for a flexible interconnection with parallel (1→3)-β-D-glucan polymers, and/or as a point of attachment for proteins. Based on these observations we propose new approaches to how (1→6)-β-linked side chains interconnect with neighboring glucan polymers in a manner that maximizes fungal cell wall strength, while also allowing for flexibility, or plasticity. Public Library of Science 2011-11-11 /pmc/articles/PMC3214063/ /pubmed/22096604 http://dx.doi.org/10.1371/journal.pone.0027614 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Lowman, Douglas W.
West, Lara J.
Bearden, Daniel W.
Wempe, Michael F.
Power, Trevor D.
Ensley, Harry E.
Haynes, Ken
Williams, David L.
Kruppa, Michael D.
New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title_full New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title_fullStr New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title_full_unstemmed New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title_short New Insights into the Structure of (1→3,1→6)-β-D-Glucan Side Chains in the Candida glabrata Cell Wall
title_sort new insights into the structure of (1→3,1→6)-β-d-glucan side chains in the candida glabrata cell wall
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214063/
https://www.ncbi.nlm.nih.gov/pubmed/22096604
http://dx.doi.org/10.1371/journal.pone.0027614
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