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Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid
BACKGROUND: Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. However, pol β has not been purified or enzymatically characterized from the model fish species Danio rerio (zebrafish). We used...
Autores principales: | Ishido, Tomomi, Yamazaki, Naoshi, Ishikawa, Mitsuru, Hirano, Ken |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214136/ https://www.ncbi.nlm.nih.gov/pubmed/22018137 http://dx.doi.org/10.1186/1475-2859-10-84 |
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