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Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification

BACKGROUND: Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially i...

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Autores principales: Danic-Tchaleu, Gwenaelle, Heurtebise, Serge, Morga, Benjamin, Lapègue, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214155/
https://www.ncbi.nlm.nih.gov/pubmed/21989403
http://dx.doi.org/10.1186/1756-0500-4-400
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author Danic-Tchaleu, Gwenaelle
Heurtebise, Serge
Morga, Benjamin
Lapègue, Sylvie
author_facet Danic-Tchaleu, Gwenaelle
Heurtebise, Serge
Morga, Benjamin
Lapègue, Sylvie
author_sort Danic-Tchaleu, Gwenaelle
collection PubMed
description BACKGROUND: Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially in the Bivalvia. During the last few years, there have been significant increases in the number of complete mitochondrial sequences available. For bivalves, 35 complete mitochondrial genomes are now available in GenBank, a number that has more than doubled in the last three years, representing 6 families and 23 genera. In the current study, we determined the complete mtDNA sequence of O. edulis, the European flat oyster. We present an analysis of features of its gene content and genome organization in comparison with other Ostrea, Saccostrea and Crassostrea species. RESULTS: The Ostrea edulis mt genome is 16 320 bp in length and codes for 37 genes (12 protein-coding genes, 2 rRNAs and 23 tRNAs) on the same strand. As in other Ostreidae, O. edulis mt genome contains a split of the rrnL gene and a duplication of trnM. The tRNA gene set of O. edulis, Ostrea denselamellosa and Crassostrea virginica are identical in having 23 tRNA genes, in contrast to Asian oysters, which have 25 tRNA genes (except for C. ariakensis with 24). O. edulis and O. denselamellosa share the same gene order, but differ from other Ostreidae and are closer to Crassostrea than to Saccostrea. Phylogenetic analyses reinforce the taxonomic classification of the 3 families Ostreidae, Mytilidae and Pectinidae. Within the Ostreidae family the results also reveal a closer relationship between Ostrea and Saccostrea than between Ostrea and Crassostrea. CONCLUSIONS: Ostrea edulis mitogenomic analyses show a high level of conservation within the genus Ostrea, whereas they show a high level of variation within the Ostreidae family. These features provide useful information for further evolutionary analysis of oyster mitogenomes.
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spelling pubmed-32141552011-11-12 Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification Danic-Tchaleu, Gwenaelle Heurtebise, Serge Morga, Benjamin Lapègue, Sylvie BMC Res Notes Research Article BACKGROUND: Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially in the Bivalvia. During the last few years, there have been significant increases in the number of complete mitochondrial sequences available. For bivalves, 35 complete mitochondrial genomes are now available in GenBank, a number that has more than doubled in the last three years, representing 6 families and 23 genera. In the current study, we determined the complete mtDNA sequence of O. edulis, the European flat oyster. We present an analysis of features of its gene content and genome organization in comparison with other Ostrea, Saccostrea and Crassostrea species. RESULTS: The Ostrea edulis mt genome is 16 320 bp in length and codes for 37 genes (12 protein-coding genes, 2 rRNAs and 23 tRNAs) on the same strand. As in other Ostreidae, O. edulis mt genome contains a split of the rrnL gene and a duplication of trnM. The tRNA gene set of O. edulis, Ostrea denselamellosa and Crassostrea virginica are identical in having 23 tRNA genes, in contrast to Asian oysters, which have 25 tRNA genes (except for C. ariakensis with 24). O. edulis and O. denselamellosa share the same gene order, but differ from other Ostreidae and are closer to Crassostrea than to Saccostrea. Phylogenetic analyses reinforce the taxonomic classification of the 3 families Ostreidae, Mytilidae and Pectinidae. Within the Ostreidae family the results also reveal a closer relationship between Ostrea and Saccostrea than between Ostrea and Crassostrea. CONCLUSIONS: Ostrea edulis mitogenomic analyses show a high level of conservation within the genus Ostrea, whereas they show a high level of variation within the Ostreidae family. These features provide useful information for further evolutionary analysis of oyster mitogenomes. BioMed Central 2011-10-12 /pmc/articles/PMC3214155/ /pubmed/21989403 http://dx.doi.org/10.1186/1756-0500-4-400 Text en Copyright ©2011 Lapègue et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Danic-Tchaleu, Gwenaelle
Heurtebise, Serge
Morga, Benjamin
Lapègue, Sylvie
Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title_full Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title_fullStr Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title_full_unstemmed Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title_short Complete mitochondrial DNA sequence of the European flat oyster Ostrea edulis confirms Ostreidae classification
title_sort complete mitochondrial dna sequence of the european flat oyster ostrea edulis confirms ostreidae classification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214155/
https://www.ncbi.nlm.nih.gov/pubmed/21989403
http://dx.doi.org/10.1186/1756-0500-4-400
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