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Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering

BACKGROUND AND METHODS: In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differen...

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Autores principales: Hajiali, Hadi, Shahgasempour, Shapour, Naimi-Jamal, M Reza, Peirovi, Habibullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215154/
https://www.ncbi.nlm.nih.gov/pubmed/22114477
http://dx.doi.org/10.2147/IJN.S24312
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author Hajiali, Hadi
Shahgasempour, Shapour
Naimi-Jamal, M Reza
Peirovi, Habibullah
author_facet Hajiali, Hadi
Shahgasempour, Shapour
Naimi-Jamal, M Reza
Peirovi, Habibullah
author_sort Hajiali, Hadi
collection PubMed
description BACKGROUND AND METHODS: In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mechanical properties were also measured by the tensile test. Furthermore, for biocompatibility assessment, human umbilical vein endothelial cells and human umbilical artery smooth muscle cells were cultured on these scaffolds, and cell attachment and viability were evaluated. RESULTS: PGA with 10 wt% gelatin enhanced the endothelial cells whilst PGA with 30 wt% gelatin increased smooth muscle cell adhesion, penetration, and viability compared with the other scaffold blends. Additionally, with the increase in gelatin content, the mechanical properties of the scaffolds were improved due to interaction between PGA and gelatin, as revealed by Fourier transform infrared spectroscopy and differential scanning calorimetry. CONCLUSION: Incorporation of gelatin improves the biological and mechanical properties of PGA, making promising scaffolds for vascular tissue engineering.
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spelling pubmed-32151542011-11-23 Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering Hajiali, Hadi Shahgasempour, Shapour Naimi-Jamal, M Reza Peirovi, Habibullah Int J Nanomedicine Original Research BACKGROUND AND METHODS: In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mechanical properties were also measured by the tensile test. Furthermore, for biocompatibility assessment, human umbilical vein endothelial cells and human umbilical artery smooth muscle cells were cultured on these scaffolds, and cell attachment and viability were evaluated. RESULTS: PGA with 10 wt% gelatin enhanced the endothelial cells whilst PGA with 30 wt% gelatin increased smooth muscle cell adhesion, penetration, and viability compared with the other scaffold blends. Additionally, with the increase in gelatin content, the mechanical properties of the scaffolds were improved due to interaction between PGA and gelatin, as revealed by Fourier transform infrared spectroscopy and differential scanning calorimetry. CONCLUSION: Incorporation of gelatin improves the biological and mechanical properties of PGA, making promising scaffolds for vascular tissue engineering. Dove Medical Press 2011 2011-09-27 /pmc/articles/PMC3215154/ /pubmed/22114477 http://dx.doi.org/10.2147/IJN.S24312 Text en © 2011 Hajiali et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Hajiali, Hadi
Shahgasempour, Shapour
Naimi-Jamal, M Reza
Peirovi, Habibullah
Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title_full Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title_fullStr Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title_full_unstemmed Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title_short Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
title_sort electrospun pga/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215154/
https://www.ncbi.nlm.nih.gov/pubmed/22114477
http://dx.doi.org/10.2147/IJN.S24312
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