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Evaluation of the magnetic field requirements for nanomagnetic gene transfection

The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity...

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Detalles Bibliográficos
Autores principales: Fouriki, A., Farrow, N., Clements, M.A., Dobson, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: CoAction Publishing 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215215/
https://www.ncbi.nlm.nih.gov/pubmed/22110859
http://dx.doi.org/10.3402/nano.v1i0.5167
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author Fouriki, A.
Farrow, N.
Clements, M.A.
Dobson, J.
author_facet Fouriki, A.
Farrow, N.
Clements, M.A.
Dobson, J.
author_sort Fouriki, A.
collection PubMed
description The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. CONCLUSION: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™.
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spelling pubmed-32152152011-11-22 Evaluation of the magnetic field requirements for nanomagnetic gene transfection Fouriki, A. Farrow, N. Clements, M.A. Dobson, J. Nano Rev Short Communication The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. CONCLUSION: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™. CoAction Publishing 2010-07-09 /pmc/articles/PMC3215215/ /pubmed/22110859 http://dx.doi.org/10.3402/nano.v1i0.5167 Text en © 2010 A. Fouriki et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Fouriki, A.
Farrow, N.
Clements, M.A.
Dobson, J.
Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title_full Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title_fullStr Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title_full_unstemmed Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title_short Evaluation of the magnetic field requirements for nanomagnetic gene transfection
title_sort evaluation of the magnetic field requirements for nanomagnetic gene transfection
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215215/
https://www.ncbi.nlm.nih.gov/pubmed/22110859
http://dx.doi.org/10.3402/nano.v1i0.5167
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