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Evaluation of the magnetic field requirements for nanomagnetic gene transfection
The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
CoAction Publishing
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215215/ https://www.ncbi.nlm.nih.gov/pubmed/22110859 http://dx.doi.org/10.3402/nano.v1i0.5167 |
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author | Fouriki, A. Farrow, N. Clements, M.A. Dobson, J. |
author_facet | Fouriki, A. Farrow, N. Clements, M.A. Dobson, J. |
author_sort | Fouriki, A. |
collection | PubMed |
description | The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. CONCLUSION: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™. |
format | Online Article Text |
id | pubmed-3215215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | CoAction Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-32152152011-11-22 Evaluation of the magnetic field requirements for nanomagnetic gene transfection Fouriki, A. Farrow, N. Clements, M.A. Dobson, J. Nano Rev Short Communication The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. METHODS: non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. RESULTS: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. CONCLUSION: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000™. CoAction Publishing 2010-07-09 /pmc/articles/PMC3215215/ /pubmed/22110859 http://dx.doi.org/10.3402/nano.v1i0.5167 Text en © 2010 A. Fouriki et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Fouriki, A. Farrow, N. Clements, M.A. Dobson, J. Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title | Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title_full | Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title_fullStr | Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title_full_unstemmed | Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title_short | Evaluation of the magnetic field requirements for nanomagnetic gene transfection |
title_sort | evaluation of the magnetic field requirements for nanomagnetic gene transfection |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215215/ https://www.ncbi.nlm.nih.gov/pubmed/22110859 http://dx.doi.org/10.3402/nano.v1i0.5167 |
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