Cargando…
EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation–division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that i...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215724/ https://www.ncbi.nlm.nih.gov/pubmed/22110668 http://dx.doi.org/10.1371/journal.pone.0027542 |
_version_ | 1782216427761041408 |
---|---|
author | Jorge, Ana M. Hoiczyk, Egbert Gomes, João P. Pinho, Mariana G. |
author_facet | Jorge, Ana M. Hoiczyk, Egbert Gomes, João P. Pinho, Mariana G. |
author_sort | Jorge, Ana M. |
collection | PubMed |
description | EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation–division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis. |
format | Online Article Text |
id | pubmed-3215724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32157242011-11-21 EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus Jorge, Ana M. Hoiczyk, Egbert Gomes, João P. Pinho, Mariana G. PLoS One Research Article EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation–division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis. Public Library of Science 2011-11-14 /pmc/articles/PMC3215724/ /pubmed/22110668 http://dx.doi.org/10.1371/journal.pone.0027542 Text en Jorge et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jorge, Ana M. Hoiczyk, Egbert Gomes, João P. Pinho, Mariana G. EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus |
title | EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
|
title_full | EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
|
title_fullStr | EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
|
title_full_unstemmed | EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
|
title_short | EzrA Contributes to the Regulation of Cell Size in Staphylococcus aureus
|
title_sort | ezra contributes to the regulation of cell size in staphylococcus aureus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3215724/ https://www.ncbi.nlm.nih.gov/pubmed/22110668 http://dx.doi.org/10.1371/journal.pone.0027542 |
work_keys_str_mv | AT jorgeanam ezracontributestotheregulationofcellsizeinstaphylococcusaureus AT hoiczykegbert ezracontributestotheregulationofcellsizeinstaphylococcusaureus AT gomesjoaop ezracontributestotheregulationofcellsizeinstaphylococcusaureus AT pinhomarianag ezracontributestotheregulationofcellsizeinstaphylococcusaureus |